标签归档：马来

预活化马来酰亚胺-AAT Bioquest荧光染料

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预活化马来酰亚胺 价格 1386
产品规格

300 ug

产品货号

预活化马来酰亚胺

产品参数
Ex (nm) – Em (nm) –
分子量 – 溶剂 –
存储条件 在零下15度以下保存
产品概述

产品基本信息

产品名称：预活化马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

 

产品介绍

ReadiUse 预活化马来酰亚胺是硫醇反应性HRP衍生物。它可用于容易记具有硫醇基团的蛋白质（例如抗体）或其他生物分子。ReadiUse 预活化HRP马来酰亚胺坚固且易于使用。它可以用于通过简单混合标记抗体，即抗体的微酸性或中性溶液（pH6-7）可以直接与HRP马来酰亚胺混合，并将反应混合物摇动1-2小时。在大多数情况下，所得溶液可直接用于ELISA测定而无需进一步纯化。金畔生物是AAT Bioquest的中国代理商，为您提供优质的预活化马来酰亚胺。 

 

参考文献

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Amber Milton
Journal: (2017)

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Junjie Chi, Bingbing Gao, Mi Sun, Fengling Zhang, Enben Su, Hong Liu, Zhongze Gu
Journal: Analytical Chemistry (2017)

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Hassan Albadawi, John W Chen, Rahmi Oklu, Yue Wu, Gregory Wojtkiewicz, Benjamin Pulli, John D Milner, Richard P Cambria, Michael T Watkins
Journal: Radiology (2016): 152222

Myeloperoxidase Nuclear Imaging for Epileptogenesis
Authors: Yinian Zhang, Daniel P Seeburg, Benjamin Pulli, Gregory R Wojtkiewicz, Lionel Bure, Wendy Atkinson, Stefan Schob, Yoshiko Iwamoto, Muhammad Ali, Wei Zhang
Journal: Radiology (2015): 822–830

Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Benjamin Pulli, Muhammad Ali, Yoshiko Iwamoto, Matthias WG Zeller, Stefan Schob, Jenny J Linnoila, John W Chen
Journal: Antioxidants & redox signaling (2015): 1255–1269

Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
Authors: Jiansheng Huang, Forrest Smith, Peter Panizzi
Journal: Archives of biochemistry and biophysics (2014): 74–85

Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings
Authors: Frank D Scherag, Thomas Brandstetter, Jürgen Rühe
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576–582

Measuring myeloperoxidase activity in biological samples
Authors: Benjamin Pulli, Muhammad Ali, Reza Forghani, Stefan Schob, Kevin LC Hsieh, Gregory Wojtkiewicz, Jenny J Linnoila, John W Chen
Journal: PLoS One (2013): e67976

Micro-volume wall-less immunoassays using patterned planar plates
Authors: Katherine R Kozak, Jianyong Wang, Melvin Lye, Rashi Takkar, Namyong Kim, Hyunjae Lee, Noo Li Jeon, Kedan Lin, Crystal Zhang, Wai Lee T Wong
Journal: Lab on a Chip (2013): 1342–1350

Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imaging
Authors: Anne Kleijn, John W Chen, Jason S Buhrman, Gregory R Wojtkiewicz, Yoshiko Iwamoto, Martine L Lamfers, Anat O Stemmer-Rachamimov, Samuel D Rabkin, Ralph Weissleder, Robert L Martuza
Journal: Clinical Cancer Research (2011): 4484–4493

5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺 CAS 154480-30-5-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺 CAS 154480-30-5 价格 2823
产品规格

1 mg

产品货号

5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺 CAS 154480-30-5

产品参数
Ex (nm) 552 Em (nm) 578
分子量 481.51 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺

CAS：154480-30-5 

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：481.51

溶剂：DMSO

激发波长（nm）：552

发射波长（nm）：578

 

产品介绍

5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺是美国AAT Bioquest生产的罗丹明类染料。四甲基罗丹明-5-马来酰亚胺是从四甲基罗丹明-5(6)-马来酰亚胺混合物中纯化出来的单体，广泛地用于多肽和蛋白质的硫醇修饰。在一些复杂的生物学研究中，实验比材料耗费更加关键。与6-异构体相比，四甲基罗丹明-5-马来酰亚胺更适合用于蛋白质和多肽的硫羟化修饰。金畔生物是AAT Bioquest的中国代理商，为您提供优质的5-TAMRA 马来酰亚胺 四甲基罗丹明-5-马来酰亚胺。 

点击查看光谱

 

参考文献

4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)

Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533

Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338

Cytochrome c conformations resolved by the photon counting histogram: watching the alkaline transition with single-molecule sensitivity
Authors: Perroud TD, Bokoch MP, Zare RN.
Journal: Proc Natl Acad Sci U S A (2005): 17570

Membrane topology of loop 13-14 of the Na+/glucose cotransporter (SGLT1): a SCAM and fluorescent labelling study
Authors: Gagnon DG, Holt A, Bourgeois F, Wallendorff B, Coady MJ, Lapointe JY.
Journal: Biochim Biophys Acta (2005): 173

Perturbation analysis of the voltage-sensitive conformational changes of the Na+/glucose cotransporter
Authors: Loo DD, Hirayama BA, Cha A, Bezanilla F, Wright EM.
Journal: J Gen Physiol (2005): 13

The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505

Distances between tropomyosin sites across the muscle thin filament using luminescence resonance energy transfer: evidence for tropomyosin flexibility
Authors: Chen Y, Lehrer SS.
Journal: Biochemistry (2004): 11491

Conformational dynamics of Na+/K+- and H+/K+-ATPase probed by voltage clamp fluorometry
Authors: Geibel S, Zimmermann D, Zifarelli G, Becker A, Koenderink JB, Hu YK, Kaplan JH, Friedrich T, Bamberg E.
Journal: Ann N Y Acad Sci (2003): 31

6-OG488 马来酰亚胺（相当于Oregon Green® 488 马来酰亚胺）-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
6-OG488 马来酰亚胺（相当于Oregon Green® 488 马来酰亚胺）价格 2823
产品规格

5 mg

产品货号

6-OG488 马来酰亚胺（相当于Oregon Green® 488 马来酰亚胺）

产品参数
Ex (nm) 498 Em (nm) 526
分子量 463.35 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：6-OG488 马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：463.35

溶剂：DMSO

激发波长（nm）：496

发射波长（nm）：524

 

产品介绍

6-OG488 马来酰亚胺是美国AAT Bioquest生产的荧光染料。OG488马来酰亚胺与OregonGreen®488马来酰亚胺（Invitrogen＃O6034，OregonGreen®是Invitrogen的商标）是相同的分子。这种荧光素的氟化类似物具有较高的光稳定性和较低的pKa~4.7，而荧光素马来酰亚胺具有pKa~6.4。OG488马来酰亚胺的pKa降低使其荧光在生理pH范围内基本上对pH不敏感。 该硫醇反应性OG488马来酰亚胺可用于制备亮绿色荧光生物共轭物，其激发/发射大值为~496 / 524nm。 它是具有严重pH依赖性和低光稳定性的荧光素马来酰亚胺的极好替代品。金畔生物是AAT Bioquest的中国代理商，为您提供优质的6-OG488 马来酰亚胺。 

点击查看光谱

 

参考文献

Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506

Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579

A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654

The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581

Cetuximab-Oregon Green 488
Authors: Unknown
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)

Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Zandvoort M.
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB.
Journal: Anal Biochem (2002): 18

Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151

蛋白质和硫醇化生物分子的马来酰亚胺标记

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蛋白质和其他硫醇化生物分子的马来酰亚胺标记

硫醇与马来酰亚胺的反应是广泛用于生物共轭和标记生物分子（包括蛋白质和肽）的过程。它按照以下方案进行：

蛋白质和硫醇化生物分子的马来酰亚胺标记

马来酰亚胺是对硫醇表现出高选择性的亲电子化合物。虽然马来酰亚胺在自然界中几乎不存在，但硫醇却非常丰富。它们以半胱氨酸残基形式存在于蛋白质和肽中。尽管天然DNA不含硫醇，但具有硫醇基团的合成寡核苷酸可以很容易地制备。

硫醇易于氧化二聚并形成二硫键。因此，半胱氨酸残基形成二硫键，从而稳定蛋白质的三级结构。二硫化物不与马来酰亚胺反应。因此，有必要在缀合前还原二硫化物并排除反应中的氧气。

缀合方案取决于起始组分的溶解度。对于水溶性较低的化合物（如大多数荧光染料马来酰亚胺），必须使用有机共溶剂（如 DMSO或 DMF）。

我们建议采用以下方案将Lumprobe 染料马来酰亚胺与蛋白质、肽和其他硫醇化生物分子结合。

  1. 将待标记的蛋白质或其他含有硫醇的分子溶解在塑料小瓶中的脱气缓冲液中，pH 7-7.5（PBS、Tris 和 HEPES 很好，但也可以使用其他不含硫醇的缓冲液）。可以通过在缓冲液上施加真空几分钟或通过惰性气体（氮气、氩气或氦气）鼓泡来对缓冲液进行脱气。对于蛋白质，最佳浓度为 1-10 mg/mL。

  2. 添加过量的 TCEP（三羧乙基膦）试剂以减少二硫键，用惰性气体冲洗并关闭。 TCEP 过量 100 倍就可以了。将混合物在室温下放置 20 分钟。

  3. 将马来酰亚胺染料溶解在 DMSO 或新鲜 DMF 中（100 uL 中 1-10 mg）。

  4. 将马来酰亚胺染料溶液添加到硫醇溶液中（染料过量 20 倍），用惰性气体冲洗小瓶，然后紧紧关闭。

  5. 充分混合并在 4°C 或室温下放置过夜。

  6. 通过凝胶过滤、HPLC、FPLC 或电泳纯化产物。

对于水溶性差的马来酰亚胺，像大多数染料马来酰亚胺一样，我们建议使用共溶剂（DMF 或 DMSO）。具有良好水溶性的马来酰亚胺（如磺基-氰基马来酰亚胺）可以溶解在水中。如果出现沉淀，请增加混合物中有机助溶剂的含量，以实现更好的标记。

建议仅将透析作为纯化具有良好水溶性的马来酰亚胺的方法。

Cy3 马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cy3 马来酰亚胺价格 2823
产品规格

1 mg

产品货号

Cy3 马来酰亚胺

产品参数
Ex (nm) 555 Em (nm) 569
分子量 866.92 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：Cy3,马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：866.92

溶剂：DMSO

激发波长（nm）：554

发射波长（nm）：568

 

产品介绍

Cy3 马来酰亚胺是美国AAT Bioquest生产的荧光染料，各种花青3（Cy3）染料已用于标记生物分子，用于荧光成像和其他基于荧光的生化分析。 它们广泛用于标记肽，蛋白质和寡核苷酸等。Cy3染料在与蛋白质结合后荧光大幅度增强，Cy3NHS酯易与氨基反应。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Cy3 马来酰亚胺。 

点击查看光谱

点击查看实验方案

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

 

相关产品

产品名称 货号
Cy3 胺 Cat#145
Cy3 叠氮化物 Cat#143
Cy3 炔烃 Cat#144

Cy3.5 马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cy3.5 马来酰亚胺价格 4245
产品规格

1 mg

产品货号

Cy3.5 马来酰亚胺

产品参数
Ex (nm) 579 Em (nm) 591
分子量 1198.24 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：Cy3.5 马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：1198.24

溶剂：DMSO

激发波长（nm）：581

发射波长（nm）：596

 

产品介绍

Cy3.5 马来酰亚胺是美国AAT Bioquest生产的荧光染料，花菁染料Cy3.5，常被应用于生物分子标记，荧光成像及其他荧光生物分析。他们广泛应用于多肽，蛋白，核苷酸等。Cy3.5染料在与蛋白质结合后荧光大幅度增强。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Cy3.5 马来酰亚胺。 

点击查看光谱

点击查看实验方案

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

 

相关产品

产品名称 货号
Cy3.5 酸 Cat#147
Cy3.5 胺 Cat#139
Cy3.5 炔烃 Cat#982

Cy5 马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cy5 马来酰亚胺价格 2823
产品规格

1 mg

产品货号

Cy5 马来酰亚胺

产品参数
Ex (nm) 651 Em (nm) 670
分子量 892.96 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：Cy5,马来酰亚胺

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：892.96

溶剂：DMSO

激发波长（nm）：650

发射波长（nm）：669

 

产品介绍

Cy5 马来酰亚胺是美国AAT Bioquest生产的荧光染料，各种花青5（Cy5）染料已用于标记生物分子，用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽，蛋白质和寡核苷酸等。Cy5染料是常见的红色荧光团中的一种。这些多功能荧光团可以耐受3-10的pH范围，从而可以用于生物相关的各种pH应用。该染料还具有DMSO耐受性和光稳定性，能够在不损失性能的情况下从储存转移至分析。水溶性在测定缓冲液中对有机溶剂的需要。我们的Cy5荧光染料经过QC测试，可确保高水平的发色团和活性染料含量。单活性染料适用于蛋白质和寡核苷酸的靶向，标记，双活性染料更适用于一般标记。建议使用NHS酯染料标记胺基，建议使用马来酰亚胺染料标记硫醇基团。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Cy5 马来酰亚胺。 

点击查看光谱

点击查看实验方案

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

 

相关产品

产品名称 货号
Cy5 胺 Cat#155
Cy5 叠氮化物 Cat#153
Cy5 酰肼 Cat#156

Cy7 马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cy7 马来酰亚胺价格 3534
产品规格

1 mg

产品货号

Cy7 马来酰亚胺

产品参数
Ex (nm) 756 Em (nm) 779
分子量 918.99 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：Cy7,马来酰亚胺

储存条件：-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：918.99

溶剂：DMSO

激发波长(nm)：749

发射波长(nm)：776

 

产品介绍

Cy7 马来酰亚胺是美国AAT Bioquest生产的Cy系列染料，各种花青7（Cy7）染料已用于标记生物分子，用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽，蛋白质和寡核苷酸等。Cy7染料缀合物是体内成像应用中常用的近红外红色荧光团的一种。Cy7NHS酯易与氨基反应。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Cy7 马来酰亚胺。 

点击查看光谱

点击查看实验方案

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

 

相关产品

产品名称 货号
Cy7 胺 Cat#165
Cy7 酰肼 Cat#166
Cy7 叠氮化物 Cat#163

Cy5.5 马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cy5.5 马来酰亚胺价格 3534
产品规格

1 mg

产品货号

Cy5.5 马来酰亚胺

产品参数
Ex (nm) 683 Em (nm) 703
分子量 1153.20 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：Cy5.5 马来酰亚胺

储存条件：保存在冰箱-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：1153.20

溶剂：DMSO

激发波长(nm)：682

发射波长(nm)：702

 

产品介绍

Cy5.5 马来酰亚胺是美国AAT Bioquest生产的Cy系列染料。 花菁染料Cy5.5，常被应用于生物分子标记，荧光成像及其他荧光生物分析。他们广泛应用于多肽，蛋白，核苷酸等。Cy5.5染料在与蛋白质结合后荧光大幅度增加。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Cy5.5 马来酰亚胺。 

点击查看光谱

点击查看实验方案

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

 

相关产品

产品名称 货号
Cy5.5 胺 Cat#176
Cy5.5 叠氮化物 Cat#178
Cy5.5 酰肼 Cat#177

地高辛马来酰亚胺-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
地高辛马来酰亚胺价格 2823
产品规格

1 mg

产品货号

地高辛马来酰亚胺

产品参数
Ex (nm) – Em (nm) –
分子量 570.68 溶剂 DMSO
存储条件 在零下15度以下保存
产品概述

产品基本信息

产品名称：地高辛马来酰亚胺

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：570.68

外观：固体

溶剂：DMSO

 

产品介绍

地高辛马来酰亚胺是美国AAT Bioquest生产的荧光染料，地高辛配基（DIG）是一种具有高抗原性的小分子半抗原，与其他流行的半抗原（如2,4-二硝基苯酚（DNP）和生物素）相似，它在许多生物学检测中都被使用。通常，地高辛与要检测的大生物分子（例如蛋白质，核酸等）缀合。抗地高辛配基抗体直接或二次用荧光染料或酶标记，以进行可视化和检测。地高辛配基马来酰亚胺在温和条件下容易与巯基反应，将地高辛配基部分连接到蛋白质或硫醇取代的寡核苷酸上。地高辛配基标记的生物分子与高亲和力和特异性的抗地高辛配基抗体结合用于生物检测。金畔生物是AAT Bioquest的中国代理商，为您提供优质的地高辛马来酰亚胺。 

 

试剂应用文献

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
 
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
 
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
 
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
 
Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
Authors: Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.
Journal: Methods Mol Biol (2017): 199-210
 
Synthesis of MeON-neoglycosides of digoxigenin with 6-deoxy- and 2,6-dideoxy-d-glucose derivatives and their anticancer activity
Authors: Wang, D. D., Li, X. S., Bao, Y. Z., Liu, J., Zhang, X. K., Yao, X. S., Sun, X. L., Tang, J. S.
Journal: Bioorg Med Chem Lett (2017): 3359-3364
 
Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity
Authors: Lai, T. P., Wright, W. E., Shay, J. W.
Journal: Biotechniques (2016): 306-9
 
Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
Authors: Wu, W., Gong, P., Li, J., Yang, J., Zhang, G., Li, H., Yang, Z., Zhang, X.
Journal: RNA (2014): 580-4
 
Elimination of nonspecific bands in non-radioactive electrophoretic mobility shift assays using the digoxigenin system
Authors: Tokunaga, S., Stegeman, J. J.
Journal: Anal Biochem (2014): 70-2
 
Digoxigenin-labeled in situ hybridization for the detection of Streptococcus suis DNA in polyserositis and a comparison with biotinylated in situ hybridization
Authors: Kang, I., Seo, H. W., Park, C., Oh, Y., Lee, J., You, O. H., Kim, S. H., Gottschalk, M., Chae, C.
Journal: J Vet Med Sci (2014): 109-12