As a researcher, you want to have as much control as possible over what goes into your study animals. At Envigo, we understand that. That is why we developed Teklad Global Rodent Diets®. Teklad Global Rodent Diets® are a special integrated range of vegetarian laboratory rodent diets developed to be nutritionally complete for various life stages from breeding through long-term maintenance. Global rodent laboratory diets contain levels of protein, energy, vitamins and minerals that are more appropriate to the needs of modern biomedical research studies.
Furthermore, in global rodent diets particular attention has been placed on avoiding, as far as practical, ingredients that are reported to have adverse confounding effects on experimental results. This has resulted in a range of Teklad rodent laboratory diets that contain:
No fish meal
No meat meals or meat by-products
No alfalfa meal
No soybean meal or reduced levels
No animal fat
By excluding animal by-products, the presence of nitrosamines (a potential carcinogen) is avoided. Exclusion of alfalfa meal reduces chlorophyll, improving optical imaging clarity. Reduction or removal of soybean meal, together with elimination of alfalfa meal, minimizes levels of naturally-occurring phytoestrogens. Phytoestrogens interact with endogenous estrogens and potentially can affect studies in many research areas. Read more about this in these pieces:
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.
Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer global diet line for your modern research needs.
Teklad Global Rodent Diets® are modern formulas designed to reduce research variables. Specifically, these diets contain more appropriate nutrient levels, and limit or exclude ingredients that are reported to have effects on a wide variety of research endpoints.
Lower, more appropriate protein levels can improve survival and reduce morbidity
Vegetarian with no nitrosamines (a potential carcinogen)
Formulated to exclude alfalfa meal, greatly improving fluorescent imaging clarity
Formulated to exclude or lower soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
Extruded rodent diets dramatically reduce clumping and hardness after autoclaving (2018SX, 2019S, 2020SX), and in general result in less diet waste and cleaner cages
Teklad rodent diets are natural-ingredient diets specifically formulated to provide the proper balance of all known nutrients considered essential for the growth, maintenance, and reproduction of rats, mice, gerbils and hamsters. These diets conform to the nutrient requirements for rodents established by the National Research Council (1995).
Teklad rodent diets provide uniform nutrition. They are fixed-formula diets designed to minimize the nutrient variances which otherwise could occur if the ingredient composition of a diet were altered from one batch to the next.
Protein is supplied primarily by plant sources. Supplemental amino acids are added to provide the proper amount and balance of essential amino acids. All rodent diets are fortified with vitamins and minerals to help support the regulation of body fluids and the proper functioning of body systems to ensure the adequate growth, maintenance, and reproduction of research rodents. Autoclavable diets are supplemented with additional vitamins to compensate for losses that occur during autoclaving. Since our diets are nutritionally complete and balanced, it is not necessary to add dietary supplements.
There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.
Recommended storage conditions:
Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
Clean and free of pests
In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air
This product is for research use only. Do not administer it to human. Antibodies against Macrophage/Microglia-specific Protein Iba1 Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.
Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1. Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers. Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1. Working Concentration: Immunocytochemistry 1-2 μg/mL Storage:Store at -20°C. After opening, aliquot contents and freeze at -20°C. Package Size: 50 μg (100 μL) Specificity：
Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1. References:参考文献
Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).
Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).
Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).
Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).
Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).
iba1小胶质细胞抗体 免疫印迹⇒ 016-20001 (50μg (100μL))
wako Anti Iba1, Rabbit 016-20001 (for Western Blotting)
Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
Developed at Hampton Research
PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated. PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Crystals of Peptidoglycan Recognition Protein (PGRP) from camel milk grown using the Hampton Research PEG/Ion screen. Sharma, P., Singh, N., Sinha, M., Sharma, S., Perbandt, M., Betzel, C., Kaur, P., Srinivasan, A. & Singh, T.P. All India Institute of Medical Sciences, Department of Biophysics, New Delhi, India.
Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet. The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet). The resulting mixture recreated in TD.88051/TD.90221 (same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage. Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases. Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats. Contact us for more information, modifications, or possible control diets.
Examples of hybrid high-fat diets with added cholesterol and cholate source*:
TD.88051 and TD.90221 (same formula) are Teklad product codes for hybrid atherogenic diets
Example of hybrid high-fat diet with added cholesterol (without cholate source):
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Also used for lithogenic (gallstone) rodent studies. Key dietary features:
75% rodent breeder diet; 25% purified ingredients
High fat (~15% by weight; 37% kcal from fat)
Saturated fatty acids (SFA >45% of total fatty acids)
Cholate source (0.5%)*
Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.
Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res, 2000. 41(4): p. 521-31.
George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65. Arterioscler Thromb Vasc Biol, 1999. 19(3): p. 505-10.
Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol, 2002. 22(1): p. 121-6.
Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis, 1987. 68(3): p. 231-40.
Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis, 1998. 136(1): p. 17-24.
Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J Biol Chem, 2003. 278(44): p. 42774-84.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See TD.96121 for a purified diet and TD.94059 for a hybrid diet. Contact us for additional options.