分类目录归档：未分类

ENDURO™迷你电泳电源

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品牌：美国Labnet

订货号：E0304-230V

产品介绍

      Labnet全新设计的EnduroTM 迷你电泳电源是DNA、RNA、蛋白电泳和印迹转移电泳的理想选择。

      迷你电泳电源相比同系列的250V/300V电源，身材更小巧更节省实验室空间，拥有更高的性价比，可额外购买配套的电源箱，使实验更高效并节约空间。

         ENDURO™迷你电泳电源      ENDURO™迷你电泳电源

      电源的微处理器采用反馈回路信号控制以保持操作参数的稳定。 程序选择包括恒定的电压输出和恒定的电流输出，定时或连续的运行。设定值采用轻触薄膜键完成，数字显示。一些安全参数已经被设计到软件中包括自动断路、空载探测、瞬间载荷变化探测和过电压保护。如果发生断电，可以设置机器重启。2个输出终端可以允许2对不同的凝胶电泳同时进行。

 

型号

Model 250V

Model 300V

Mini

货号

E0203-230V

E0303-230V

E0304

输出类型

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

输出电压范围/增量

5-250V/1V

2-300V/1V

10-300V/1V

输出电流范围/增量

10-3,000mA/10mA

4-500mA/1mA

10-400mA/1mA

输出功率范围/增量

1-300W/1W

90W max/NA

60W

定时器

1min～99hr59min/连续

1min～99hr59min/连续

1-999min带报警/连续

编程功能

有，10步编程，可存20个程序

无

无

显示 

液晶显示屏

液晶显示屏

液晶数字显示屏

可连接电泳系统数量

4个

4个

2个

尺寸

19x25x8cm

19x25x8cm

14×19.1×8.4 cm

重量

2.5kg

2.2kg

1kg

输入电源

230V~50Hz

230V~50Hz

100-240V

 

  • 上海金畔生物科技有限公司

    文章号：558-558

Harlan teklad官网动物饲料 Rodent diets 啮齿动物饮食

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Harlan teklad官网动物饲料 Rodent diets 啮齿动物饮食
作为一名研究人员，你想要有尽可能多的控制进入你的研究动物。在恩维戈，我们理解。这就是为什么我们开发了特克拉德全球啮齿动物饮食®.
特克拉德全球啮齿动物饮食®是一个特殊的综合素食实验室啮齿动物的饮食发展为营养完整的各个生命阶段，从繁殖到长期维持。全球啮齿动物实验室饮食中含有更适合现代生物医学研究需要的蛋白质、能量、维生素和矿物质水平。
此外，在全球啮齿类饮食中，人们特别注意尽可能避免使用据报对实验结果产生不利影响的成分。这导致了一系列特克拉德啮齿动物实验室饮食，其中包括：

  • 无鱼粉
  • 无肉食或肉类副产品
  • 无苜蓿粉
  • 不含豆粕或降低水平
  • 无动物脂肪

通过排除动物副产品，避免了亚硝胺(一种潜在的致癌物)的存在.紫花苜蓿粉的排除降低了叶绿素，提高光学成像清晰度。减少或去除豆粕，加上消除紫花苜蓿粉，可使自然产生的植物雌激素水平降至最低。植物雌激素与内源性雌激素相互作用，可能对许多研究产生影响。研究领域。在以下几篇文章中可以了解更多关于这一点的内容：

产品
特克勒德啮齿类食物 不可蒸压式 高压釜 辐照
总蛋白14% 2014年c 2014年s 2914
总蛋白16% 2016年c 2016年s 2916
Teklead全局18%蛋白 2018年c 2018S，2018SX 2918
TKLAD全局19%蛋白挤出 2019 2019 S 2919
全无大豆蛋白挤出 2020 X 2020 SX 2920 X
传统饮食 见更多
并不是所有的产品都是在当地库存的，可能需要延长交货期和额外的费用。
许多饮食都是由符合产品代码的“C”指定的认证格式提供的。当饮食被认证时，一个有代表性的样本会被测试一组污染物。如果没有经过认证的库存，可以根据要求提供认证。可适用最低订货量和附加费用。
折叠所有标签

传统的啮齿动物饮食是在几十年前根据对啮齿动物的营养、成分和当时的饮食制造的理解而制定的。虽然传统的饮食将提供已知的您的实验室动物的营养需求，我们建议您考虑使用我们的新的全球饮食线的饮食，以满足您的现代研究需要。
特克拉德全球啮齿动物饮食®现代公式旨在减少研究变量。具体来说，这些饮食含有更合适的营养水平，限制或排除据报道对各种研究终点有影响的成分。

  • 更低、更合适的蛋白质水平可以提高存活率和降低发病率。
  • 不含亚硝胺的素食(一种潜在的致癌物)
  • 除紫花苜蓿粉外，可大大提高荧光成像清晰度。
  • 用来排除或降低豆粕，从而减少异黄酮的存在。异黄酮是实验室动物饮食中发现的植物雌激素的主要类型。
  • 挤压后的啮齿类食物能显着地减少压蒸后的结块和硬度。2018SX, 2019 S, 2020 SX)，并且通常导致更少的饮食垃圾和更干净的笼子
特克勒德啮齿动物饮食是一种特殊的天然成分饮食，旨在提供所有已知营养的适当平衡，这些营养素被认为对老鼠、沙土鼠和仓鼠的生长、维持和繁殖至关重要。这些饮食符合国家研究委员会对啮齿类动物的营养要求(1995年)。
特克拉德啮齿动物的饮食提供了统一的营养。它们是一种固定的配方奶饮食，旨在尽量减少营养差异，否则，如果一种饮食的成分组成从一批改变到下一批，就会发生这种变化。
蛋白质主要由植物来源提供。补充氨基酸是为了提供适当的数量和平衡的必需氨基酸。所有啮齿动物的饮食都含有维生素和矿物质，以帮助支持体液的调节和身体系统的正常运作，以确保研究鼠的充分生长、维持和繁殖。可蒸压饮食补充维生素，以弥补在高压灭菌过程中发生的损失。由于我们的饮食是营养完整和平衡的，所以没有必要添加膳食补充剂。
没有一个确定的点，人们能够预测什么时候一个特定的饮食会变质或变得缺乏一个或多个营养。六个月保质期的共同准则是以北美长期的实践为基础的。在欧洲和亚洲，当地做法和监管监督方面的差异导致特克拉德标准天然成分饮食被例行使用到9个月，有时甚至在生产后12个月。这种实践经验，随着时间的推移，再加上文献支持和维生素测试，让我们相信，这些饮食继续支持动物的健康和研究的完整性，至少在9个月后生产。如果你不确定当地的政策，请向你的机构寻求指导。
建议的储存条件：

  • 凉爽和干燥；在华氏70度或以下，湿度最好在50%以下，但高达65%是可以接受的。
  • 清洁无害虫
  • 在原始包装中或容器中，防止连续暴露于光线下，并将空气暴露在最小范围内。
标准的天然成分啮齿类食物是大鼠、老鼠、沙土鼠和仓鼠的完整饮食，这是应该允许的。[医]锂节食的机会。任何时候都应该有淡水供应。

As a researcher, you want to have as much control as possible over what goes into your study animals. At Envigo, we understand that. That is why we developed Teklad Global Rodent Diets®.
Teklad Global Rodent Diets® are a special integrated range of vegetarian laboratory rodent diets developed to be nutritionally complete for various life stages from breeding through long-term maintenance. Global rodent laboratory diets contain levels of protein, energy, vitamins and minerals that are more appropriate to the needs of modern biomedical research studies.
Furthermore, in global rodent diets particular attention has been placed on avoiding, as far as practical, ingredients that are reported to have adverse confounding effects on experimental results. This has resulted in a range of Teklad rodent laboratory diets that contain:

  • No fish meal
  • No meat meals or meat by-products
  • No alfalfa meal
  • No soybean meal or reduced levels
  • No animal fat

By excluding animal by-products, the presence of nitrosamines (a potential carcinogen) is avoided. Exclusion of alfalfa meal reduces chlorophyll, improving optical imaging clarity. Reduction or removal of soybean meal, together with elimination of alfalfa meal, minimizes levels of naturally-occurring phytoestrogens. Phytoestrogens interact with endogenous estrogens and potentially can affect studies in many research areas. Read more about this in these pieces:

Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 14% protein 2014, 2014C 2014S 2914
Teklad global 16% protein 2016, 2016C 2016S 2916
Teklad global 18% protein 2018, 2018C 2018S, 2018SX 2918
Teklad global 19% protein extruded 2019 2019S 2919
Teklad global soy protein-free extruded 2020X 2020SX 2920X
Traditional diets see more
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.
COLLAPSE ALL TABS

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer global diet line for your modern research needs.
Teklad Global Rodent Diets® are modern formulas designed to reduce research variables. Specifically, these diets contain more appropriate nutrient levels, and limit or exclude ingredients that are reported to have effects on a wide variety of research endpoints.

  • Lower, more appropriate protein levels can improve survival and reduce morbidity
  • Vegetarian with no nitrosamines (a potential carcinogen)
  • Formulated to exclude alfalfa meal, greatly improving fluorescent imaging clarity
  • Formulated to exclude or lower soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Extruded rodent diets dramatically reduce clumping and hardness after autoclaving (2018SX, 2019S, 2020SX), and in general result in less diet waste and cleaner cages
Teklad rodent diets are natural-ingredient diets specifically formulated to provide the proper balance of all known nutrients considered essential for the growth, maintenance, and reproduction of rats, mice, gerbils and hamsters. These diets conform to the nutrient requirements for rodents established by the National Research Council (1995).
Teklad rodent diets provide uniform nutrition. They are fixed-formula diets designed to minimize the nutrient variances which otherwise could occur if the ingredient composition of a diet were altered from one batch to the next.
Protein is supplied primarily by plant sources. Supplemental amino acids are added to provide the proper amount and balance of essential amino acids. All rodent diets are fortified with vitamins and minerals to help support the regulation of body fluids and the proper functioning of body systems to ensure the adequate growth, maintenance, and reproduction of research rodents. Autoclavable diets are supplemented with additional vitamins to compensate for losses that occur during autoclaving. Since our diets are nutritionally complete and balanced, it is not necessary to add dietary supplements.
There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.
Recommended storage conditions:

  • Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
  • Clean and free of pests
  • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air
Standard natural ingredient rodent diets are complete diets for rats, mice, gerbils and hamsters, that should be allowed ad libitum access to the diet. Fresh water should be available at all times.

wako Anti Iba1, Rabbit小胶质细胞/巨噬细胞特异性抗体

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wako Anti Iba1, Rabbit小胶质细胞/巨噬细胞特异性抗体

先关产品：

wako Anti Iba1, Rabbit For Immunocytochemistry)

wako Anti Iba1, Rabbit  016-20001 (for Western Blotting)

Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体

        钙离子是已知的所有细胞，包括中枢神经系统（CNS）细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动，其中许多钙结合蛋白被划分成一个大的蛋白家族，EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白，在巨噬细胞和小胶质细胞中表达，并在这些细胞的活化过程中表达升高。
本抗体以合成的的Iba1羧基末端序列为抗原，该序列是人，大鼠和小鼠Iba1蛋白质保守序列。本抗体（免疫组化用）特异性与小胶质细胞/巨噬细胞反应，免疫双重染色与单克隆抗体结合，可与GFAP单克隆抗体（(胶质纤维酸性蛋白，Glial Fibrillary Acidic Protein）联合使用对脑组织和细胞培养物进行双重染色。

特点

S特异性检测小胶质细胞与/巨噬细胞，不与神经细胞与星形胶质细胞发生交叉反应。可与人，大鼠，小鼠的Iba1反应

Wako 016-26721 Anti Iba1, Monoclonal Antibody(NCNP24) for Immunochemistry 50ul
Wako 012-26723 Anti Iba1, Monoclonal Antibody(NCNP24) for Immunochemistry 10ul
Wako 013-26471 Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated for Immunochemistry 100ul
小胶质细胞/巨噬细胞特异性蛋白抗体(结合红色荧光素635)
Wako 016-26461 Anti Iba1, Rabbit, Biotin-conjugated for Immunochemistry 100ul
小胶质细胞/巨噬细胞特异性蛋白抗体（结合生物素）
Wako  019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) for Immunochemistry 50μg
小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化)
Wako 016-20001 Anti Iba1, Rabbit (for Western Blotting) for Immunochemistry 50μg
小胶质细胞/巨噬细胞特异性蛋白抗体(免疫印迹)

应用

石蜡切片应用方法：
1. 固定
2. 用0.01M PBS缓冲液清洗二次
3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
4. 用0.01M PBS缓冲液清洗三次
5. 使用封闭液（含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液）室温孵育2小时
6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
7. 用0.01M PBS缓冲液清洗三次
8. 使用含有生物素标记的抗兔IgG（1：200）的封闭液室温孵育1小时
9. 用0.01M PBS缓冲液清洗三次
10. 使用Elite ABC试剂（含有试剂A1：50，试剂B1：50的0.01M PBS溶液）
11. 用0.01M PBS缓冲液清洗三次

12. 过氧化物酶溶液（含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液）

巨噬细胞/小胶质细胞特异性蛋白抗体Iba1的应用

免疫细胞化学⇒和光 Cat. No. 019-19741 (50μg (100μL))
说明书下载地址

http://www.siyaku.com/uh/Flh.do?file_path=/ANTI_FILES/W01/W01W0101-1974/D0010.pdf&now=1481080836480

免疫印迹⇒和光Cat. No. 016-20001 (50μg (100μL))
众所周知，钙离子是中枢神经系统细胞信号传递的重要介质。钙离子通过与各种钙结合蛋白关联激活信号，其中大多数蛋白属于EF手性蛋白家族。
 Iba1属于17-kDa EF蛋白，在巨噬细胞和小胶质细胞中特异性表达，起到正向调节的作用。
和光提供的兔多抗可识别Iba1羧基末端序列的合成肽，这段Iba1蛋白序列在人、大鼠和小鼠中均存在。这些抗体可特异性的识别小胶质细胞和巨噬细胞，适用于脑组织的双重免疫染色，以及细胞培养中与GFAP单抗联合使用（GFAP单抗可特异性的识别星形胶质细胞）。
 
特征：特异性识别人、大鼠、小鼠的小胶质细胞及巨噬细胞，无法作用于神经元和星形胶质细胞。
 
大鼠原代的细胞培养的双重免疫染色和同一位置的对比图像
绿色：Iba1，小胶质细胞/巨噬细胞特异性蛋白抗体作用 (Wako Cat. #019-19741)
红色：星形胶质细胞，与GFAP单抗作用
      （此数据由 日本神经系统科学国家研究所神经化学部提供）

Iba1抗体用于免疫细胞化学(Wako Cat. #019-19741)试验过程

   石蜡切片应用
1）去石蜡
100%二甲苯 10分钟  2次
100%乙醇   5分钟     2次
80%乙醇   5分钟 
70%乙醇   5分钟 
一蒸馏水    5分钟      2次
2）用0.01M缓冲液冲洗两次；
3）在含0.3%过氧化氢的甲醇里孵育30分钟；
4））用0.01M缓冲液冲洗三次；
5）在室温下，放进含有1.5%山羊血清和1%牛血清蛋白的0.001MPBS的封闭液里孵育2小时；
6）在4度下，与 Anti Iba1 Antibody (0.5 μg/mL)(Wako Catalog No. 019‐19741)在封闭液中孵育过夜；
7）用0.01M PBS冲洗3次；
8）在室温下，与生物素标记的anti‐Rabbit IgG Antibody (1:200)在封闭液中孵育1小时；
9）用0.01M PBS冲洗3次；
10）在室温下，与ABC试剂（试剂A（1：50）和试剂B(1:50)与0.01MPBS混匀）孵育1小时
11）用0.01MPBS冲洗3次；
12)在过氧化物酶溶液（含0.01%过氧化氢，0.05%DAB的0.05MTris缓冲液）中孵育；

Anti Iba1, Rabbit (for Immunocytochemistry)   019-19741 介绍

小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化)

鉴定小胶质细胞的标记物。众多文献引用。产品质量稳定。小胶质细胞属于神经免疫细胞，在当前神经生物学研究的热点。包括从事眼科研究，神经退行性疾病的客户会用到该产品。
文献报道可用于石蜡切片和冰冻切片的免疫组化（IHC）实验。

品牌：Wako
CAS No.：
储存条件：-20℃
纯度：0.5mg/ml

详细介绍：
This product is for research use only. Do not administer it to human.
Antibodies against Macrophage/Microglia-specific Protein Iba1
Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.
Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.
Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.
Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.
Working Concentration: Immunocytochemistry 1-2 μg/mL
Storage:Store at -20°C. After opening, aliquot contents and freeze at -20°C.
Package Size: 50 μg (100 μL)
Specificity：
Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.
References:参考文献

  1. Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).
  2. Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).
  3. Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).
  4. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).
  5. Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

 

iba1小胶质细胞抗体 免疫印迹⇒ 016-20001 (50μg (100μL))

wako Anti Iba1, Rabbit  016-20001 (for Western Blotting)

钙离子作为信号转导通道中的第一信使，在所有细胞包括中枢神经细胞的生命活动中起重要作用。钙离子与不同的钙结合蛋白结合后进行级联反应。Iba1 是能与巨噬细胞和小胶质细胞发生特异性结合的钙结合蛋白，当这些细胞激活后，Iba1 的表达量上调，因此Iba1 通常作为鉴定小胶质细胞的标记物使用。近来研究发现，小胶质细胞除了提供营养、保护神经的作用外，还被证明可产生NO、TNF- α、IL-1 β等物质，因此将小胶质细胞定义为中枢神经系统中的巨噬细胞样吞噬细胞，具有重要的免疫细胞作用。当病原菌，病毒入侵中枢神经系统时会起免疫监视的作用。近来研究发现，该细胞可能与神经退行性疾病的发生和发展有关。本产品是可与小胶质细胞发生特异性反应的兔多抗，配合对星形胶质细胞有特异性的GFAP 单抗，可进行双染色。
抗原：合成肽（Iba1 的C 末端序列）
外观：TBS 溶液（1mg/ml）
提纯：抗原亲和提纯
特异性：对小胶质细胞、巨噬细胞特异反应。不会与神经元、星形胶质细胞发生交叉反应。
能与人、小鼠、大鼠Iba1 反应。
用法: ① Iba1 抗体，兔源（Immunocytochemistry 用）
适合Immunocytochemistry。
只需1 ～ 2 ug/ml 即可进行实验。
② Iba1 抗体，兔源（Western blotting 用）适合Western blotting。
只需0.5 ～ 1ug/ml 即可进行实验。

绿色：Iba1 抗体，兔源（Immunocytochemistry 用）（小胶质细胞）
红色：抗GFAP 抗体（星形胶质细胞）
数据提供：日本国立精神• 神经中心神经研究所代谢研究部

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications
  Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
Features

Developed at Hampton Research
PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
 Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.
PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT
Crystals of Peptidoglycan Recognition Protein (PGRP) from camel milk grown using the Hampton Research PEG/Ion screen. Sharma, P., Singh, N., Sinha, M., Sharma, S., Perbandt, M., Betzel, C., Kaur, P., Srinivasan, A. & Singh, T.P. All India Institute of Medical Sciences, Department of Biophysics, New Delhi, India.

CAT NO NAME DESCRIPTION
HR2-126 PEG/Ion Screen 10 ml, tube format
HR2-098 PEG/Ion 2 Screen 10 ml, tube format
HR2-139 PEG/Ion HT 1 ml, Deep Well block format

ENDURO™迷你电泳电源

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品牌：美国Labnet

订货号：E0304-230V

产品介绍

      Labnet全新设计的EnduroTM 迷你电泳电源是DNA、RNA、蛋白电泳和印迹转移电泳的理想选择。

      迷你电泳电源相比同系列的250V/300V电源，身材更小巧更节省实验室空间，拥有更高的性价比，可额外购买配套的电源箱，使实验更高效并节约空间。

         ENDURO™迷你电泳电源      ENDURO™迷你电泳电源

      电源的微处理器采用反馈回路信号控制以保持操作参数的稳定。 程序选择包括恒定的电压输出和恒定的电流输出，定时或连续的运行。设定值采用轻触薄膜键完成，数字显示。一些安全参数已经被设计到软件中包括自动断路、空载探测、瞬间载荷变化探测和过电压保护。如果发生断电，可以设置机器重启。2个输出终端可以允许2对不同的凝胶电泳同时进行。

 

型号

Model 250V

Model 300V

Mini

货号

E0203-230V

E0303-230V

E0304

输出类型

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

恒定电压或恒定电流，自动短路保护

输出电压范围/增量

5-250V/1V

2-300V/1V

10-300V/1V

输出电流范围/增量

10-3,000mA/10mA

4-500mA/1mA

10-400mA/1mA

输出功率范围/增量

1-300W/1W

90W max/NA

60W

定时器

1min～99hr59min/连续

1min～99hr59min/连续

1-999min带报警/连续

编程功能

有，10步编程，可存20个程序

无

无

显示 

液晶显示屏

液晶显示屏

液晶数字显示屏

可连接电泳系统数量

4个

4个

2个

尺寸

19x25x8cm

19x25x8cm

14×19.1×8.4 cm

重量

2.5kg

2.2kg

1kg

输入电源

230V~50Hz

230V~50Hz

100-240V

 

  • 上海金畔生物科技有限公司

    文章号：558-558

harlan teklad官网 TD.88051和TD.90221 TD.94059 胆固醇和胆酸盐的混合高脂饮食

发表于由
Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet. The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet). The resulting mixture recreated in TD.88051/TD.90221 (same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage. Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases. Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats. Contact us for more information, modifications, or possible control diets.
Examples of hybrid high-fat diets with added cholesterol and cholate source*:

  • TD.88051 and TD.90221 (same formula) are Teklad product codes for hybrid atherogenic diets

Example of hybrid high-fat diet with added cholesterol (without cholate source):

Research Use:
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Also used for lithogenic (gallstone) rodent studies.
Key dietary features:

  • 75% rodent breeder diet; 25% purified ingredients
  • High fat (~15% by weight; 37% kcal from fat)
  • Saturated fatty acids (SFA >45% of total fatty acids)
  • Cholesterol (1.25%)
  • Cholate source (0.5%)*

References:

  1. Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.
  2. Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res, 2000. 41(4): p. 521-31.
  3. George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65. Arterioscler Thromb Vasc Biol, 1999. 19(3): p. 505-10.
  4. Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol, 2002. 22(1): p. 121-6.
  5. Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis, 1987. 68(3): p. 231-40.
  6. Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis, 1998. 136(1): p. 17-24.
  7. Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J Biol Chem, 2003. 278(44): p. 42774-84.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See TD.96121 for a purified diet and TD.94059 for a hybrid diet. Contact us for additional options.