NISSUI 日本日水培养基 08630 FASTKIT SLIM EGG “Nissui”
NISSUI 日本日水培养基 08630 FASTKIT SLIM EGG “Nissui”
水溶性lumiprobe荧光染料C53B0 Sulfo-Cyanine7 alkyne 10 mg 水溶性lumiprobe荧光染料Sulfo-Cyanine7 炔基 10 mg 470
种属反应性
Amresco 0512-100G POTASSIUM IODIDE 碘化钾
日本进口动物安全标记笔Animal Marker
上海金畔生物科技有限公司提供5202一次性小鼠灌胃针 M340444
日本进口小鼠软管一次性灌胃针
5202一次性小鼠灌胃针 M340444 详情
日本进口Ptfe软管材质 一次性小鼠灌胃针
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此灌胃针使用Ptfe软管材质,前端附有硅胶软球。可以有效减少对肺部以及邻近器官的损伤。操作简便。
适用于多种小型实验动物,规格齐全,更可根据您的需求尺寸进行定制。
5根配套小包装,EOG灭菌。
固话总机:021-50837765
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网 址: www.jinpanbio.com
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com
更多 www.jinpanbio.com.cn www.utopbio.com
lumiprobe荧光染料3C041 Cyanine5 NHS ester minimal dye 25 nmol lumiprobe荧光染料Cyanine5 羧基活性酯 minimal dye 25 nmol 210
该文章由WP-AutoPost插件自动采集发布
品牌:美国Labnet
订货号:TC9610-230V
产品介绍
Labnet新款MultiGene Optimax PCR仪带来了新的运行速度和功能。该款机器继承了MultiGene原先简单的操作界面,并拥有更快的升降温速度,大大减少反应过程的运行时间。增加了PCR viewer和TM计算器等功能。PCR viewer通过USB接口与仪器连接使用,透过PCR viewer窗口,可以实时查看实际的温度变化曲线。全新采用了6 – 4×4孔peltier排列格式,将整个反应模块划分成6个独立的区域,可以在每方阵设置独立的温度进行最佳条件摸索,或者进行不同温度的PCR反应。你不须选择高温和低温,而是让系统完全按照你的要求设置,为研究提供更大的灵活性。 Labnet MultiGene Optimax PCR仪,以实惠的价格给您带来卓越的性能。
二、特点
● 快速升降温速度。
● 热盖压力恒定,滑盖防止烫伤;可立便于清洁。
● 热盖2种温度范围可选。
● 全新热盖设计,高度防止冷凝水设计。
● 6个独立控制的Peltier 模块。
上海金畔生物科技有限公司 文章号:441-441 康宁corning 3621 TUBE,MICENT,1.7ML,NAT,SC,NS,B, 透明,带有PU覆盖层的螺旋状镀锌钢丝加强 -40°C/+ 90°C(短时间可耐受125°C) 聚醚型聚氨酯软管, 带有PU覆盖层的螺旋状镀锌钢丝加强筋。内壁平滑以保证物料在管内流动顺畅。壁厚均为1.5毫米。耐霉菌、耐水解。具有优异的耐臭氧和耐候性能。符合FDA, EU10/2011。该软管无毒、无味、不含卤素,符合RoHS环保指令的要求。螺旋钢丝接地可以排静电,符合TRB S 2153的规定。阻燃度符合UL 94 HB标准。 食品加工行业、制药行业、割草机和扫地清洁车等需要耐霉菌和耐水解的应用。可用于油脂类食品的输送。
产品名称:PL-024 食品级1.5mm物料PU软管 中国证券监督管理委员会(证监会)上市公司并购重组审核委员会在今日上午召开的第19次会议中,审核通过了成都天兴仪表股份有限公司发行股份购买资产的相关事宜,标志着基因测序领先企业贝瑞和康借壳上市取得了实质性的成功。 下面我们来系统回顾本次并购重组过程中的几个重要阶段: 2016年6月 成都天兴仪表股份有限公司发布停牌公告,透漏公司将筹划重大资产重组事项,至此,开始长达6个月的停牌过程。 2016年8月 天兴仪表的重大资产方案首次披露:天兴仪表将其目前拥有的全部资产、负债、业务、人员等出售给天兴集团或其指定的第三方,天兴集团或其指定的第三方以现金形式购买;同时,天兴仪表向北京贝瑞和康生物技术股份有限公司全体股东发行股份购买其持有的贝瑞和康100%股权。重组完成后,上市公司将持有贝瑞和康100%股权,贝瑞和康借壳天兴仪表,实现A股上市。但关于具体的重组方案并未披露。 2016年12月 天兴仪表披露重大资产重组草案,拟以21.14元/股,发行股份2.03亿股,作价43亿元购买贝瑞和康100%股权。同时向大股东天兴集团的控股子公司,2.97亿元出售上市公司资产与负债。交易完成后,基因测序企业贝瑞和康将借壳上市。同时披露2013年、2014年、2015年和2016年上半年,贝瑞和康主营业务收入分别为2.58亿元、3.34亿元、4.45亿元和3.85亿元,增长态势良好。同期,医学产品及服务业务贡献的毛利分别为1.6亿元、2.08亿元、2.38亿元和2.43亿元,占贝瑞和康主营业务毛利额的比例分别为90.51%、89.04%、88.63%和97.29%,为贝瑞和康利润的最主要来源。业绩补偿方承诺2017年、2018年、2019年的净利润分别不低于2.284亿元、3.092亿元和4.045亿元。天兴仪表股票于12月19日复牌后,迎来连续10个涨停板。 2017年1月 证监会向天兴仪表寄出《行政许可项目审查一次反馈意见通知书》,并对此次借壳重组事项连续提出33个问题,要求天兴仪表作出书面说明和解释,并在30个工作日内向证监会提交书面回复意见。 2017年2月 天兴仪表股价创出了近一个月的新高46.86元。2月28日,该股票被停牌一天。同时,该公司因 2015年、2016年连续两个会计年度经审计的净利润为负值,根据《深圳证券交易所股票上市规则》的相关规定,深圳证券交易所有权对公司股票交易实行“退市风险警示”。股票简称由“天兴仪表”变更为“*ST天仪”,实行退市风险警示后公司股票交易的日涨跌幅限制为 5%。 2017年3月 *ST天仪(000710)于3月9日发布了一份长达131页篇幅,约十万字的答复书,公布了一系列有关贝瑞和康的相关数据,包括近些年销售业绩、与主要竞争对手的基本情况对比分析,以及答复证监会此前下发的《中国证监会行政许可项目审查一次反馈意见通知书》等内容。3月21日,证监会又向天兴仪表寄出《行政许可项目审查二次反馈意见通知书》,并对此次借壳重组事项继续提出3个问题。 2017年4月 4月12日,*ST天仪控股股东天兴集团所持有的44,002,000股股份(占总股本的29.10%)被四川省高级人民法院冻结,并于4月22日解除冻结。期间,为了回复证监会的二次反馈意见,天兴仪表再次调整贝瑞和康重组方案,将鼎锋明德致知、鼎锋明德正心已经将其所持贝瑞和康1,563,672股股份全部转让给君联茂林、苏州启明创智、鼎锋海川。4月20日,证监会发布《并购重组委2017年第19次工作会议公告》,定于2017年4月26日上午9:00召开2017年第19次并购重组委工作会议,其中审核的并购重组申请人包括成都天兴仪表股份有限公司。4月26日,证监会上市公司并购重组审核委员会召开了第19次会议,审核通过了成都天兴仪表股份有限公司发行股份购买资产的相关事宜,标志着基因测序领先企业贝瑞和康借壳上市取得了实质性的成功! 该文章由WP-AutoPost插件自动采集发布 Amresco 0706蛋白酶 K lumiprobe荧光染料47070 Cyanine5.5 hydrazide 25 mg lumiprobe荧光染料Cyanine5.5 酰肼 25 mg 410 该文章由WP-AutoPost插件自动采集发布 甘油检测试剂盒 分析物意义:常见食品组分,或作为甜味剂,或用于改善口感 Megazyme检测试剂盒优点:新型的药片模式,性质更稳定,反应快 The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials. UV-method for the determination of Glycerol in foodstuffs, Principle: (pyruvate kinase) (L-lactate dehydrogenase) Kit size: 70 assays (manual) / 700 (microplate) Advantages Q1. There is an issue with the performance of the kit; the results are not as expected. If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following: K-GCROL is highly specific for glycerol. Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results. The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit. Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit. It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed, by dilution (if required) in distilled water. The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2 The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation. The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can. There a 3 main methods for calculation of results using the microplate format: Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range. The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected. For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit): 1. The kit components are listed on pages 2-3 of the kit booklet. To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples. If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor). The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume). Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered. For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results. The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes. However, the level of accuracy is obviously analyst and sample dependent. Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay. Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay. Cell disruption is only require to measure glycerol within microbial cells. To measure glycerol in the extracellular media only then cell disruption is not required and centrifugation of the sample may be sufficient, e.g.: (a) Determination of glycerol in cell culture media/supernatants. In general, the concentration of glycerol in cell culture media/supernatants can be determined without any sample treatment (except clarification by centrifugation/filtering or dilution according to the dilution table, if necessary). Typically, no clarification or dilution is required, and a sample volume of 0.1 mL is satisfactory. If interference is suspected then sample clarification/deproteinisation using carrez reagents or perchloric acid should be used (methods are provided in the kit booklet).
康宁corning 3621 TUBE,MICENT,1.7ML,NAT,SC,NS,B,
离心管 1.7ml 自然色 弹扣盖(snap cap) 未灭菌 散装 500个/包
离心管 1.7ml 自然色 弹扣盖(snap cap) 未灭菌 散装 500个/包 10包/箱 4028.06PL-024 食品级1.5mm物料PU软管 – 食品、医药行业应用
颜色
温度
特性
应用
产品编码
内径
壁厚
耐负压
弯曲半径
体积
重量
包装长度
No.
in.
mm
mm
mH2O
mm
m3
kg/m
m
PL-024-1000
1″
25
1.5
9.5
30
0.041
0.305
20
PL-024-1250
1-1/4″
32
1.5
9.5
35
0.056
0.435
20
PL-024-1500
1-1/2″
38
1.5
9.5
40
0.074
0.465
20
PL-024-1580
1-9/16″
40
1.5
9.5
50
0.077
0.485
20
PL-024-1770
*
45
1.5
9.0
50
0.090
0.545
20
PL-024-1970
*
50
1.5
9.0
60
0.099
0.600
20
PL-024-2000
2″
51
1.5
9.0
60
0.106
0.610
20
PL-024-2360
2-3/8″
60
1.5
8.5
70
0.122
0.710
20
PL-024-2500
2-1/2″
63
1.5
8.0
70
0.148
0.745
20
PL-024-2560
2-9/16″
65
1.5
7.5
70
0.152
0.765
20
PL-024-2760
*
70
1.5
7.5
80
0.165
0.920
20
PL-024-2950
*
75
1.5
7.0
80
0.176
0.985
20
PL-024-3000
3″
76
1.5
7.0
90
0.232
0.995
20
PL-024-3150
*
80
1.5
6.0
90
0.254
1.045
20
PL-024-3540
*
90
1.5
5.0
100
0.294
1.170
20
PL-024-3940
*
100
1.5
4.0
120
0.281
1.295
15
PL-024-4000
4″
102
1.5
3.5
120
0.286
1.320
15
PL-024-4330
*
110
1.5
3.2
130
0.307
1.420
15
PL-024-4720
*
120
1.5
3.0
140
0.333
1.545
15
PL-024-5000
5″
127
1.5
2.9
150
0.405
2.015
15
PL-024-5120
*
130
1.5
2.6
150
0.414
2.065
15
PL-024-5510
*
140
1.5
2.4
160
0.504
2.215
15
PL-024-5910
*
150
1.5
2.0
170
0.537
2.370
15
PL-024-6000
6″
152
1.5
2.0
170
0.544
2.400
15
PL-024-6300
*
160
1.5
1.8
180
0.662
2.525
15
PL-024-7090
*
180
1.5
1.5
210
0.855
2.580
15
PL-024-7870
*
200
1.5
1.2
230
1.130
2.860
15
PL-024-8000
8″
203
1.5
1.2
230
1.146
2.900
15
PL-024-8980
*
228
1.5
1.0
260
0.933
3.250
10
PL-024-9840
*
250
1.5
0.8
290
1.170
3.555
10
PL-024-10000
10″
254
1.5
0.8
290
1.188
3.610
10
PL-024-10430
*
265
1.5
0.7
300
1.238
3.765
10
PL-024-11000
11″
279
1.5
0.7
320
1.301
3.960
10
PL-024-12000
12″
305
1.5
0.7
350
1.618
5.245
10
行业应用:PL-024 食品级1.5mm物料PU软管 应用透明,带有PU覆盖层的螺旋状镀锌钢丝加强尘埃落定!贝瑞和康借壳上市获证监会无条件通过
Amresco 0706蛋白酶 K
中文名称:蛋白酶K
CAS号:39450-01-6
级别:Chromatographically purified
分子量:~18kDa
Activity (hemoglobin,pH 7.5; 37 °C):≥ 30mAnsonU/mg
Specific activity:≥40mAnsom u/mg protein
DNases (Nicking activity,pBR 322,6 h,37℃):Not detectable
RNases (RNA,2 h,37℃):Not detectable
Solubility in water:(20 ℃) soluble
pH value:6.2~6.8 (10 g/l,H2O,20℃)
蛋白酶K 性状:白色或类白色粉末,是一种非特异性、枯草杆菌蛋白酶相关的丝氨酸蛋白酶, 来源于白色念球菌(. Tritirachium album. ),具有很高的比活性。EDTA等鳌合剂或SDS等去垢剂不能使蛋白酶K失活,反而增强和稳定酶活性,并在较广的PH范围内(PH4-12.5)均有活性。在组织或细胞核酸分离时,使核酸酶(RNA和DNA)以及反应中其他酶失活。
蛋白酶K 用途:生化研究。广谱蛋白酶,95C 加热10 分钟,则完全失活。可替代 DEPC 处理 RNA 抽提用的离心管和枪头,实现灭活 RNase A 的目的,也用于处理 RNase-Free的水。充分降解组织或细胞中蛋白质分子特别是核酸,以达到释放和萃取高质量核酸的辅助试剂。用于质粒或基因组DNA、RNA的分离以及蛋白多肽印迹实验。
保存:2~8℃
蛋白酶K 化学试剂的种类很多,世界各国对化学试剂的分类和分级的标准不尽一致。
IUPAC对化学标准物质的分类为:
A级:原子量标准。
B级:和A 级最接近的基准物质。
C级:含量为100+-0.02%的标准试剂
D级:含量为100+-0.05%的标准试剂
E级:以C级或D级为标准对比测定得到的纯度的试剂
化学试剂按用途可分为标准试剂,一般试剂,生化试剂等等。
我国习惯将相当于IUPAC 的C级、D级的试剂称为标准试剂。
荧光染料47070 Cyanine5.5 hydrazide 25 mg
学校供给饮食相关_脂肪残留物性试验用试剂|简易分析|一般分析纯试剂|试剂|关东化学株式会社
甘油检测试剂盒 K-GCROL 70 assays (manual) / 700 assays (microplate)
英文名:Glycerol Assay Kit
货号:K-GCROL
规格:70 assays (manual) / 700 assays (microplate)
市场价: 1808元
Suitable for manual and microplate formats.
beverages and other materials
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(2) ADP + PEP → ATP + pyruvate
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK
FAQ解答
Q2. Is K-GCROL specific for glycerol?
Some compounds that are known not to react or interfere with the assay include:
Polyethylene glycol
Ethylene glycol
Propylene glycolQ3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Q4. Should the pH of the sample be adjusted even for samples in acidic media?
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.Q5. Is the Glycerol Assay Kit (K-GCROL) suitable for measurement using cell culture media samples?
Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?
Q7. Can the manual assay format be scaled down to a 96-well microplate format?
Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.Q9. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?
Q10. Can you explain, step by step, how to follow the method and perform the kit assay?
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.Q11. I have some doubts about the appearance/quality of a kit component what should be done?
Q12. How much sample should be used for the clarification/extraction of my sample?
Q13. Can the sensitivity of the kit assay be increased?
Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?
Q15. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.
Q16. To measure fermentation samples contain high microbial cell density, is cell disruption required?