5(6)羧基四甲基罗丹明马来酰亚胺 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
5(6)羧基四甲基罗丹明马来酰亚胺 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*价格 2823
产品规格

5 mg

产品货号

5(6)羧基四甲基罗丹明马来酰亚胺 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*

产品参数
Ex (nm) 552 Em (nm) 578
分子量 481.51 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:5(6)羧基四甲基罗丹明马来酰亚胺

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:481.51

溶剂:DMSO

激发波长(nm):552

发射波长(nm):578

 

产品介绍

5(6)羧基四甲基罗丹明马来酰亚胺是美国AAT Bioquest生产的罗丹明类染料。四甲基罗丹明-5(6)-马来酰亚胺是非常好的荧光标记探针,通过硫醇修饰多肽、核苷酸、核酸,蛋白质形成具有橙色荧光的生物偶联物。马来酰亚胺是硫醇修饰常用的试剂。大多数蛋白质中,硫醇反应的位点在半胱氨酸残基上,这是蛋白质所固有的,会导致蛋白质中半胱氨酸减少。与碘乙酰胺不同,马来酰亚胺在生理条件下不与组氨酸和甲硫氨酸反应。金畔生物是AAT Bioquest的中国代理商,为您提供优质的5(6)羧基四甲基罗丹明马来酰亚胺。 

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参考文献

4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)

Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533

Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338

Cytochrome c conformations resolved by the photon counting histogram: watching the alkaline transition with single-molecule sensitivity
Authors: Perroud TD, Bokoch MP, Zare RN.
Journal: Proc Natl Acad Sci U S A (2005): 17570

Membrane topology of loop 13-14 of the Na+/glucose cotransporter (SGLT1): a SCAM and fluorescent labelling study
Authors: Gagnon DG, Holt A, Bourgeois F, Wallendorff B, Coady MJ, Lapointe JY.
Journal: Biochim Biophys Acta (2005): 173

Perturbation analysis of the voltage-sensitive conformational changes of the Na+/glucose cotransporter
Authors: Loo DD, Hirayama BA, Cha A, Bezanilla F, Wright EM.
Journal: J Gen Physiol (2005): 13

The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505

Distances between tropomyosin sites across the muscle thin filament using luminescence resonance energy transfer: evidence for tropomyosin flexibility
Authors: Chen Y, Lehrer SS.
Journal: Biochemistry (2004): 11491

Conformational dynamics of Na+/K+- and H+/K+-ATPase probed by voltage clamp fluorometry
Authors: Geibel S, Zimmermann D, Zifarelli G, Becker A, Koenderink JB, Hu YK, Kaplan JH, Friedrich T, Bamberg E.
Journal: Ann N Y Acad Sci (2003): 31

 

相关产品

产品名称 货号
5(6)-TAMRA, SE 5(6)-羧基四甲基罗丹明琥珀酰亚胺酯 Cat#370
5(6)-羧基四甲基罗丹明乙二胺 Cat#354
5(6)-羧基四甲基罗丹明尸胺 Cat#355

1mM Tetramethylrhodamine-dUTP溶液-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
1mM Tetramethylrhodamine-dUTP溶液价格 1386
产品规格

25 nmoles

产品货号

1mM Tetramethylrhodamine-dUTP溶液

产品参数
Ex (nm) 552 Em (nm) 578
分子量 1115.77 溶剂 Water
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:1mM 四甲基罗丹明-dUTP溶液

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:1115.77

溶剂:水

激发波长(nm):546

发射波长(nm):564

 

产品介绍

染料修饰的脱氧尿苷5′-三磷酸(例如氨基烯丙基dUTP)可用于通过常规酶促掺入方法(例如逆转录,切口平移,随机引物标记或PCR)产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该TMR-dUTP缀合物可用作具有Spectrum Orange®过滤器组的橙色荧光颜色(Spectrum Orange®是Vysis的商标)。

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参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953