Signal Guard HRP偶联物稳定剂-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Signal Guard HRP偶联物稳定剂价格 1386
产品规格

50 mL

产品货号

Signal Guard HRP偶联物稳定剂

产品参数
Ex (nm) Em (nm)
分子量 溶剂
存储条件 在2-8度冷藏保存
产品概述

产品基本信息

产品名称:Signal Guard HRP偶联物稳定剂

储存条件:2-8℃避光防潮

保质期:24个月

  

产品介绍

液体HRP-偶联物的主要优点在于,在溶解或者稀释冻干的或者浓缩的偶联物时了人为误差。多加或者缓冲液加的不够,都会导致分析结果的变化。同时,HRP偶联物液体的缺点在于它的内在不稳定性。我们的HRP偶联物稳定剂制备用来对准备使用的HRP-偶联物进行预稀释,有效提高其偶联物的稳定性。这种稳定剂剂型可以以1:1,000浓度存储,或者已备好直接用于分析(1:100,000)。它可以再4℃下存储24个月,在室温下存贮6个月。金畔生物是AAT Bioquest的中国代理商,为您提供优质的Signal Guard HRP偶联物稳定剂。 

 

参考文献

Inactivation of creatine kinase induced by quercetin with horseradish peroxidase and hydrogen peroxide. pro-oxidative and anti-oxidative actions of quercetin
Authors: Miura T, Muraoka S, Fujimoto Y.
Journal: Food Chem Toxicol (2003): 759

Mechanistic and molecular investigations on stabilization of horseradish peroxidase C
Authors: Schmidt A, Schumacher JT, Reichelt J, Hecht HJ, Bilitewski U.
Journal: Anal Chem (2002): 3037

Electron microscopical demonstration of horseradish peroxidase by use of tetramethylbenzidine as chromogen and sodium tungstate as stabilizer (TMB-ST method): a tracing method with high sensitivity and well preserved ultrastructural tissue
Authors: Gu Y, Chen Y, Ye L.
Journal: J Neurosci Methods (1992): 1

An ultrastructural double-labelling method: immunohistochemical localization of cell adhesion molecule L1 on HRP-labelled developing corticospinal tract axons in the rat
Authors: Joosten EA.
Journal: Histochemistry (1990): 645

The histochemical reaction of horseradish peroxidase in rodent ‘whole-brain’ preparations
Authors: Cooper AM.
Journal: Exp Brain Res (1990): 456

A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat
Authors: Dederen PJ, Joosten EA.
Journal: J Neurosci Methods (1989): 71

Improvement of the tetramethyl benzidine reaction with ammonium molybdate as a stabilizer for light and electron microscopic ligand-HRP neurohistochemistry, immunocytochemistry and double-labelling
Authors: Liang FY, Wan XC.
Journal: J Neurosci Methods (1989): 155

[Ammonium molybdate as stabilizing agent in tetramethyl benzidine reaction of CB-HRP neurohistochemistry and its application to electron microscopy and immunocytochemistry]
Authors: Liang FY, Wan XC.
Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao (1989): 142

A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP)
Authors: Olucha F, Martinez-Garcia F, Lopez-Garcia C.
Journal: J Neurosci Methods (1985): 131

Signal Guard 磷酸酶反应终止液-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Signal Guard 磷酸酶反应终止液价格 1386
产品规格

100 mL

产品货号

Signal Guard 磷酸酶反应终止液

产品参数
Ex (nm) Em (nm)
分子量 溶剂
存储条件 在2-8度冷藏保存
产品概述

Signal Guard 磷酸酶反应终止液是美国AAT Bioquest生产的用于终止磷酸酶反应的即用型终止溶液,碱性和酸性磷酸酶是分别在碱性和酸性条件下负责分子(例如核苷酸,蛋白质和生物碱)的去磷酸化的水解酶。一些磷酸酶测定和测试需要停止磷酸酶反应以使用终止溶液进行进一步分析。我们的Signal Guard 磷酸酶反应终止溶液是一种即用型试剂,可在用户确定的时间点提供终止荧光和比色信号产生磷酸酶反应的便利工具,并可使荧光或比色信号保持稳定,高可达18小时。Signal Guard 磷酸酶反应终止溶液经过优化,可与碱性,酸性和蛋白质磷酸酶相容。与其他商业磷酸酶停止试剂相比,我们的Signal Guard 磷酸酶反应终止溶液几乎是中性和温和的。它与绝大多数比色和荧光磷酸酶底物相容,而来自其他供应商的终止试剂由于其极高的pH而与大多数基于荧光的磷酸酶测定不相容。金畔生物是AAT Bioquest的中国代理商,为您提供优质的Signal Guard 磷酸酶反应终止液。 

实验方案

96孔板的测定方案
1.将Signal Guard 磷酸酶反应停止溶液温热至室温。
2.在所需的停止时间点,在每个微孔板孔中每200μL体积加入50μL的Signal Guard 磷酸酶反应终止溶液。
注意:对于其他反应体积,按比例调整Signal Guard 磷酸酶反应终止溶液的添加(例如,向25μL反应体积中加入5μL)。信号应保持稳定至少18小时。

 

示例数据分析

 

Signal Guard 磷酸酶反应终止液

图1. Signal Guard 磷酸酶反应终止溶液在酸性磷酸酶荧光(AAT Bioquest Cat#11627)测定中的应用。 通过添加200μL反应混合物引发含有100mU / mL酸性磷酸酶的两个平行反应。 将反应物在室温下温育上述时间,然后将50μLCalignGuard TM磷酸酶反应终止溶液加入到一个反应中。 该图表明Signal Guard 磷酸酶反应终止溶液完全抑制了反应。

 

参考文献

Alkaline phosphatase: a potential biomarker for stroke and implications for treatment
Authors: A. L. Brichacek
Journal: Metab Brain Dis (2019): 3-19

Discoveries of the phosphatidate phosphatase genes in yeast published in the Journal of Biological Chemistry
Authors: G. M. Carman
Journal: J Biol Chem (2019): 1681-1689

Dynamic structural rearrangements and functional regulation of voltage-sensing phosphatase
Authors: S. Sakata
Journal: J Physiol (2019): 29-40

Fat-regulating phosphatidic acid phosphatase: a review of its roles and regulation in lipid homeostasis
Authors: G. M. Carman
Journal: J Lipid Res (2019): 2-6

Myosin phosphatase: Unexpected functions of a long-known enzyme
Authors: A. Kiss
Journal: Biochim Biophys Acta Mol Cell Res (2019): 2-15

Redox inhibition of protein phosphatase PP2A: Potential implications in oncogenesis and its progression
Authors: D. Raman
Journal: Redox Biol (2019): 101105

Regulation of hematopoietic cell signaling by SHIP-1 inositol phosphatase: growth factors and beyond
Authors: M. L. Hibbs
Journal: Growth Factors (2019): 1-19

Roles of phosphatase and tensin homolog in skeletal muscle
Authors: T. Shan
Journal: J Cell Physiol (2019): 3192-3196

Structure and functions of His domain protein tyrosine phosphatase in receptor trafficking and cancer (1)
Authors: G. Desrochers
Journal: Biochem Cell Biol (2019): 68-72

The multiple functions of protein phosphatase 6
Authors: T. Ohama
Journal: Biochim Biophys Acta Mol Cell Res (2019): 74-82