标签归档：oregongreen

钙离子荧光探针OregonGreen488 BAPTA1，六钾盐-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
钙离子荧光探针OregonGreen488 BAPTA1，六钾盐价格 2823
产品规格

500 ug

产品货号

钙离子荧光探针OregonGreen488 BAPTA1，六钾盐

产品参数
Ex (nm) 493 Em (nm) 522
分子量 1114.28 溶剂 Water
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：钙离子荧光探针Oregon Green 488 BAPTA-1，六钾盐

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：1114.28

溶剂：水

激发波长(nm)：493

发射波长(nm)：522

 

产品介绍

钙离子荧光探针Oregon Green 488 BAPTA-1，六钾盐是美国AAT Bioquest生产的钙离子荧光探针，OG488 BAPTA -1与Oregon Green 488 BAPTA-1的分子相同。 它是一种可见光可激发的钙指示剂，通常与FITC过滤器组配合使用。 OG 488 BAPTA-1的激发和发射分别为494和523 nm。 结合钙离子后其荧光强度增加~14倍。 这种水溶性盐形式可用于通过显微注射或从贴片移液管输注的细胞内负载。 也可获得指示剂的细胞渗透性AM酯形式（＃20507）。金畔生物是AAT Bioquest的中国代理商，为您提供优质的钙离子荧光探针Oregon Green 488 BAPTA-1，六钾盐。 金畔生物是AAT Bioquest的中国代理商，为您提供优质的荧光素。 

点击查看光谱

钙离子篇：时间轴式讲解应用于钙离子检测的探针

 

参考文献

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339

Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J.
Journal: Am J Physiol Cell Physiol (2011): C266

Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804

Mitochondrial DNA mutations affect calcium handling in differentiated neurons
Authors: Trevelyan AJ, Kirby DM, Smulders-Srinivasan TK, Nooteboom M, Acin-Perez R, Enriquez JA, Whittington MA, Lightowlers RN, Turnbull DM.
Journal: Brain (2010): 787

钙离子荧光探针OregonGreen488 BAPTA1，AM-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
钙离子荧光探针OregonGreen488 BAPTA1，AM价格 2823
产品规格

10×50 ug

产品货号

钙离子荧光探针OregonGreen488 BAPTA1，AM

产品参数
Ex (nm) 493 Em (nm) 522
分子量 1258.07 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

OG488 BAPTA -1 AM 与 Oregon Green 488 BAPTA-1 AM 酯分子相同。它是一种细胞渗透性、可见光激发的钙指示剂，通常与 FITC 滤光片组一起使用。通过将溶解的指示剂直接添加到含有培养细胞的培养皿中，可以向细胞装载 OG488 BAPTA -1 AM。来自这些细胞的荧光信号通常使用荧光显微镜、荧光微孔板测定或流式细胞术来测量。

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适用仪器

荧光显微镜  
Ex: FITC 滤波片组
Em： FITC 滤波片组
推荐孔板: 黑色透明底板

 

流式细胞仪  
Ex: 488
Em： 530/30nm
通道: FITC

 

荧光酶标仪  
Ex: 490 nm
Em： 525 nm
Cutoff： 515 nm
推荐孔板: 黑色透明底板
读取模式: 底读模式/可分液处理

 

溶解方案(溶剂以说明书为准)

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 79.487 µL 397.434 µL 794.868 µL 3.974 mL 7.949 mL
5 mM 15.897 µL 79.487 µL 158.974 µL 794.868 µL 1.59 mL
10 mM 7.949 µL 39.743 µL 79.487 µL 397.434 µL 794.868 µL
实验方案

样品实验方案

储备溶液配制:

除非另有说明，所有未使用的储备溶液应分成一次性等份，并在制备后储存在-20°C。避免反复冻融循环。

OG488 BAPTA-1 AM

在高质量无水 DMSO 中制备 2 至 5 mM OG488 BAPTA-1 AM 储备溶液。

工作溶液配制:

OG488 BAPTA-1 AM 工作溶液

1.实验当天，将 OG488 BAPTA-1 AM 溶解在 DMSO 中，或将等份指示剂储备溶液解冻至室温。

2.在您选择的缓冲液（例如 Hanks 和 Hepes 缓冲液）中用 0.04% Pluronic® F-127 制备 2 至 20 µM OG488 BAPTA-1 AM 工作溶液。对于大多数细胞系，建议终浓度为 4-5 μM 的 Cal Green  1 AM。细胞加载所需染料的准确浓度必须根据经验确定。

注意：非离子洗涤剂 Pluronic® F-127 有时用于增加 Cal Green™ 1 AM 的水溶性。可以从金畔购买各种 Pluronic® F-127

注意：如果您的细胞含有有机阴离子转运蛋白，可将丙磺舒 (1-2 mM) 添加到染料工作溶液中（孔中的最终浓度为 0.5-1 mM），以减少脱酯后染料的外漏。各种 ReadiUse 丙磺舒产品，包括水溶性、钠盐和稳定溶液，均可从金畔购买。

 

操作步骤

以下是我们推荐的将 AM 酯加载到活细胞中的方案。该方案仅提供指导，实际应根据您的具体需求进行修改。

1.在生长培养基中将细胞孵育过夜。

2.第二天，将 1X OG488 BAPTA-1 AM 工作溶液添加到您的细胞培养板中。

3.将载有染料的板在细胞培养箱中于 37°C 下孵育 30 至 60 分钟。

注意：孵育染料超过 1 小时可以提高某些细胞系的信号强度。

4.用 HHBS 或您选择的缓冲液（包含阴离子转运蛋白抑制剂，例如 1 mM 丙磺舒，如果适用）替换染料工作溶液，以去除任何多余的探针。

5.根据需要添加刺激剂，同时使用配备 FITC 滤光片组的荧光显微镜或包含可编程液体处理系统（例如 FDSS、FLIPR 或 FlexStation）的荧光酶标仪在 Ex/Em = 490/525 nm 处测量荧光。

 

试剂应用文献

Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery
Authors: Zhang, Nisi and Guo, Yutong and Foiret, Josquin and Tumbale, Spencer K and Paulmurugan, Ramasamy and Ferrara, Katherine W
Journal: STAR protocols (2023): 102723
 
Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia
Authors: Jiang, Xiao-wen and Lu, Hong-yuan and Xu, Zi-Hua and Zhang, Ying-Shi and Zhao, Qing-Chun and others,
Journal: Fitoterapia (2021): 104824
 
Binding to carboxypeptidase M mediates protective effects of fibrinopeptide Bβ (15-42)
Authors: Sörensen-Zender, Inga and Chen, Rongjun and Song, Rong and David, Sascha and Melk, Anette and Haller, Hermann and Schmitt, Rol and , undefined
Journal: Translational Research (2019)
 
Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores
Authors: Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J
Journal: (2017)
 
Tuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores
Authors: Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J
Journal: ACS chemical biology (2017): 1844–1852

 

参考文献

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
 
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
Authors: Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.
Journal: Nat Chem Biol (2007): 423
 
An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor
Authors: Lu Y, Paige MF.
Journal: J Fluoresc (2007): 739
 
Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green
Authors: Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.
Journal: Brain Res Brain Res Protoc (2000): 132
 
Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189
 
Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran
Authors: McPherson DR, McClellan AD, O’Donovan MJ.
Journal: Brain Res Brain Res Protoc (1997): 157
 
Modulation of an Intracellular Calmodulin-Stimulated Ca2+-Pumping ATPase in Cauliflower by Trypsin (The Use of Calcium Green-5N to Measure Ca2+ Transport in Membrane Vesicles)
Authors: Askerlund P., undefined
Journal: Plant Physiol (1996): 913
 
A method for recording intracellular [Ca2+] transients in cardiac myocytes using calcium green-2
Authors: Spencer CI, Berlin JR.
Journal: Pflugers Arch (1995): 579
 
Characterization of calcium translocation across the plasma membrane of primary osteoblasts using a lipophilic calcium-sensitive fluorescent dye, calcium green C18
Authors: Lloyd QP, Kuhn MA, Gay CV.
Journal: J Biol Chem (1995): 22445
 
Calcium green-5N, a novel fluorescent probe for monitoring high intracellular free Ca2+ concentrations associated with glutamate excitotoxicity in cultured rat brain neurons
Authors: Rajdev S, Reynolds IJ.
Journal: Neurosci Lett (1993): 149