锌离子探针Zn Metal Fluor 520,钾盐-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
锌离子探针Zn Metal Fluor 520,钾盐价格 2823
产品规格

1 mg

产品货号

锌离子探针Zn Metal Fluor 520,钾盐

产品参数
Ex (nm) 493 Em (nm) 516
分子量 607.69 溶剂 Water
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:锌离子探针Zn Metal Fluor 520,钾盐

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:607.69

溶剂:水

激发波长(nm):493

发射波长(nm):516

 

产品介绍

锌离子探针Zn Metal Fluor 520,钾盐是美国AAT Bioquest生产的用于检测锌离子的荧光探针。Metal Fluor Zn-520设计用于检测突触小泡中存在的较高的锌离子浓度,并在电刺激或兴奋性毒性激动剂(0.05-50 µM)的响应下释放,并且对钙的敏感性低。Metal Fluor Zn-520在结合锌离子(> 250倍)后显示出极大的荧光增强。这种不渗透细胞的锌离子指示剂可用于检测细胞外锌离子。金畔生物是AAT Bioquest的中国代理商,为您提供优质的锌离子探针Zn Metal Fluor 520,钾盐。 

点击查看光谱

 

参考文献

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097

Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153

Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781

锌离子探针Zn Metal Fluor 520, AM-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
锌离子探针Zn Metal Fluor 520, AM价格 2823
产品规格

1 mg

产品货号

锌离子探针Zn Metal Fluor 520, AM

产品参数
Ex (nm) 493 Em (nm) 516
分子量 721.62 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

Metal Fluor Zn-520是美国AAT Bioquest生产的 用于检测突触囊泡中存在的较高锌离子浓度的锌离子探针,并响应电刺激或兴奋毒性激动剂(0.05-50μM)释放,干扰钙敏感性小。 Metal Fluor Zn-520在结合锌离子(> 250倍)后显示出很强的荧光增强作用。 该细胞渗透性AM-酯可用于检测细胞内锌离子水平。金畔生物是AAT Bioquest的中国代理商,为您提供优质的锌离子探针Zn Metal Fluor 520, AM。 

点击查看光谱

点击查看实验方案

 

底读模式

实验方案

操作步骤

1.准备HHBS缓冲液
 

2.在高质量无水DMSO中制备2 mM至5 mM Metal Fluor Zn-520,AM原液
2.1使用的金属Fluor Zn-520,AM的量:1毫克
2.2所需浓度:2 mM
2.3在合适的容器中,混合1mg的Metal Fluor TM Zn-520,AM和692.89μL的无水DMSO。
 

3.使用10μM金属Fluor Zn-520,AM 2在HHBS中制备2X工作溶液
3.1终孔内浓度为Metal Fluor™Zn-520,AM:5μM
3.2在合适的容器中混合16μL的Metal Fluor Zn-520,AM。接下来,添加HHBS或您选择的缓冲液,
注意:对于大多数细胞系,我们建议使用Metal Fluor Zn-520,AM的终浓度为4至5μM。
 

4.将100μL染料工作溶液加入已经含有100μL培养基的所需孔中
4.1该步骤将染料工作溶液从2X稀释至1X,并将每种组分的终浓度调节至以下:5μM的Metal Fluor Zn-520,AM
 

5.孵育染料
5.1将染料加载板在细胞培养箱中孵育30-60分钟。
5.2将染料加载板在室温下孵育30分钟。
 

6.准备HHBS缓冲液(或您选择的缓冲液)。
6.1在合适的容器中加入HHBS或您选择的缓冲液,直至体积为4 mL。
 

7.用HHBS缓冲液或您选择的缓冲液替换染料工作溶液,使用1.0 mM Probenecid。
7.1首先,从所需孔中除去200μL染料工作溶液和培养基。
7.2在相同的孔中加入200μL含有1.0mM丙磺舒的HHBS(或您选择的缓冲液)。
 

8.运行实验
8.1为您的样品添加所需的处理。
8.2以Ex / Em = 492/514 nm运行实验。

 

参考文献

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097

Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153

Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781