标签归档：leu

荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素] CAS 62480-44-8-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素] CAS 62480-44-8价格 2111
产品规格

10 mg

产品货号

荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素] CAS 62480-44-8

产品参数
Ex (nm) 341 Em (nm) 441
分子量 324.80 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素]

CAS：62480-44-8

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：324.80

溶剂：DMSO

激发波长(nm)：351

发射波长(nm)：430

 

产品介绍

荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素]是美国AAT Bioquest生产的荧光染料，Leu-AMC是亮氨酸氨肽酶的荧光底物。 在蛋白酶水解后，Leu-AMC释放出强烈荧光的AMC荧光团，其可以用Ex / Em = 350 / 430nm容易地检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的荧光底物Leu-AMC [L-亮氨酸-7-酰胺基-4-甲基香豆素]。 

点击查看光谱

 

参考文献

Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target
Authors: Munkhjargal T, Ishizaki T, Guswanto A, Takemae H, Yokoyama N, Igarashi I.
Journal: Vet Parasitol (2016): 14

Secretory Expression, Purification, Characterization, and Application of an Aspergillus oryzae Prolyl Aminopeptidase in Bacillus subtilis
Authors: Wang KD, Wang KH, Zhou ND, Tian YP.
Journal: Appl Biochem Biotechnol. (2016)

Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii
Authors: Lee YR, Na BK, Moon EK, Song SM, Joo SY, Kong HH, Goo YK, Chung DI, Hong Y.
Journal: PLoS One (2015): e0129884

Inhibition of leucine aminopeptidase 3 suppresses invasion of ovarian cancer cells through down-regulation of fascin and MMP-2/9
Authors: Wang X, Shi L, Deng Y, Qu M, Mao S, Xu L, Xu W, Fang C.
Journal: Eur J Pharmacol (2015): 116

Molecular Characterization of Babesia bovis M17 Leucine Aminopeptidase and Inhibition of Babesia Growth by Bestatin
Authors: Aboge GO, Cao S, Terkawi MA, Masatani T, Goo Y, AbouLaila M, Nishikawa Y, Igarashi I, Suzuki H, Xuan X.
Journal: J Parasitol (2015): 536

Modelling of human leucyl aminopeptidases for in silico off target binding analysis of potential Plasmodium falciparum leucine aminopeptidase (PfA-M17) specific inhibitors
Authors: Sahi S, Raj U, Chaudhary M, Nain V.
Journal: Recent Pat Endocr Metab Immune Drug Discov (2014): 191

Design, synthesis and preliminary activity evaluation of novel 3-amino-2-hydroxyl-3-phenylpropanoic acid derivatives as aminopeptidase N/CD13 inhibitors
Authors: Zhang X, Zhang L, Zhang J, Feng J, Yuan Y, Fang H, Xu W.
Journal: J Enzyme Inhib Med Chem (2013): 545

Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
Authors: Liew SM, Tay ST, Puthucheary SD.
Journal: BMC Microbiol (2013): 110

Exploration of structural and physicochemical requirements and search of virtual hits for aminopeptidase N inhibitors
Authors: Halder AK, Saha A, Jha T.
Journal: Mol Divers (2013): 123

Novel leucine ureido derivatives as inhibitors of aminopeptidase N (APN)
Authors: Ma C, Jin K, Cao J, Zhang L, Li X, Xu W.
Journal: Bioorg Med Chem (2013): 1621

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC价格 1386
产品规格

5 mg

产品货号

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC

产品参数
Ex (nm) 341 Em (nm) 441
分子量 498.57 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：498.57

溶剂：DMSO

激发波长(nm)：351

发射波长(nm)：430

 

产品介绍

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC是美国AAT Bioquest生产的荧光底物，弱荧光AMC基板产生亮蓝色荧光AMC产品，其具有Ex / Em = 351 / 430nm，并且可以使用DAPI滤光器组容易地检测。该AMC底物用于监测蛋白酶体的蛋白酶活性。常见的蛋白酶体形式称为26S蛋白酶体，其含有一个20S核心颗粒结构和两个19S调节帽。所有20S颗粒由四个堆叠的七聚环结构组成，它们本身由两种不同类型的亚基组成; α亚基本质上是结构性的，而β亚基主要是催化性的。堆叠中的外部两个环由每个七个α亚基组成，其用作调节颗粒的对接域，并且α亚基N末端形成阻止底物未受调节进入内腔的门。内部两个环各自由7个β亚基组成，并含有进行蛋白水解反应的蛋白酶活性位点。金畔生物是AAT Bioquest的中国代理商，为您提供优质的蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC。 

点击查看光谱

 

参考文献

Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Wenjuan Zhou, Lingling Wei, Ting Xiao, Chunyou Lai, Min Peng, Lingli Xu, Xiangwei Luo, Shaoping Deng, Fengxue Zhang
Journal: Biochemical and Biophysical Research Communications (2017): 400–406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Jennifer E Gilda, Xianyin Lai, Frank A Witzmann, Aldrin V Gomes
Journal: Molecular & Cellular Proteomics (2016): 1962–1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Rajeshwary Ghosh, Sumanta K Goswami, Luis Felipe BB Feitoza, Bruce Hammock, Aldrin V Gomes
Journal: International Journal of Cardiology (2016): 923–935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Stefanie Grimm, Christiane Ott, Melanie Hörlacher, Daniela Weber, Annika Höhn, Tilman Grune
Journal: Biochemical Journal (2012): 127–139

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 823.86 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：823.86

溶剂：DMSO

激发波长(nm)：N/A

发射波长(nm)：560

 

产品介绍

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料，这种基于氨基荧光素的探针是补体成分C3 / C5转化酶，凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它还被来自人红细胞的高分子量硫醇蛋白酶macropain水解。 可以使用我们的荧光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。 苯甲酰基-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC灵敏得多。金畔生物是AAT Bioquest的中国代理商，为您提供优质的发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
 
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

相关产品

产品名称 货号
荧光底物BOC-Leu-Gly-Arg-氨基荧光素 Cat#13001

发光底物BOC-Leu-Gly-Arg-氨基荧光素-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物BOC-Leu-Gly-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物BOC-Leu-Gly-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 819.87 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：发光底物BOC-Leu-Gly-Arg-氨基荧光素

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：819.87

溶剂：DMSO

激发波长(nm)：N/A

发射波长(nm)：560

 

产品介绍

发光底物BOC-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料，这种基于氨基荧光素的探针是补体成分C3 / C5转化酶，凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它也被来自人类红细胞的高分子量巯基蛋白酶macropain水解。 可以使用我们的萤光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。BOC-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC敏感得多。金畔生物是AAT Bioquest的中国代理商，为您提供优质的发光底物BOC-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

相关产品

产品名称 货号
荧光底物Benzoyl-Leu-Gly-Arg-氨基荧光素 Cat#13000