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BrdU 细胞增殖检测试剂 CAS 59-14-3价格 1095
产品规格
25 mg
产品货号
产品参数
| Ex (nm) | – | Em (nm) | – |
| 分子量 | 307.09 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
BrdU 细胞增殖检测试剂是美国AAT生产的用于细胞增殖的产品，染料修饰的脱氧尿苷5′-三磷酸（例如氨基烯丙基dUTP）可用于通过常规酶促掺入方法（例如逆转录，切口平移，随机引物标记或PCR）产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该TMR-dUTP缀合物可用作具有Spectrum Orange®过滤器组的橙色荧光颜色（Spectrum Orange®是Vysis的商标）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的细胞增殖检测试剂/试剂盒。
实验方案
用于染色细胞的样品方案
以下程序适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型的存在和其他因素可能影响Brdu掺入。
1）制备1-10mM DMSO储备溶液。 如果储存在-20°C，DMSO储备液可以保存6个月。
2）通过离心沉淀细胞并将细胞重悬于完全生长培养基中。 培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。
3）使用5和20μM之间的浓度添加Brdu染色并孵育60分钟过夜作为指导。 最佳孵育时间取决于细胞类型和实验目标。
4）用70-80％酒精或酸 – 乙醇固定细胞20-30分钟。
5）用PBS洗涤（3次，每次2分钟）。
6）继续进行免疫细胞化学染色技术。
参考文献
DNA interstrand crosslinks are induced in cells prelabelled with 5-bromo-2′-deoxyuridine and exposed to UVC radiation
Authors: Wojcik A, Bochenek A, Lankoff A, Lisowska H, Padjas A, Szumiel I, von Sonntag C, Obe G.
Journal: J Photochem Photobiol B (2006): 15
Authors: Wojcik A, Bochenek A, Lankoff A, Lisowska H, Padjas A, Szumiel I, von Sonntag C, Obe G.
Journal: J Photochem Photobiol B (2006): 15
Estrogen-induced region specific decrease in the density of 5-bromo-2-deoxyuridine-labeled cells in the olfactory bulb of adult female rats
Authors: Hoyk Z, Varga C, Parducz A.
Journal: Neuroscience (2006): 1919
Authors: Hoyk Z, Varga C, Parducz A.
Journal: Neuroscience (2006): 1919
Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2′-deoxyuridine or 5-bromo-2′-deoxycytidine
Authors: Zeng Y, Wang Y.
Journal: Nucleic Acids Res. (2006)
Authors: Zeng Y, Wang Y.
Journal: Nucleic Acids Res. (2006)
Exposure to 5-bromo-2′-deoxyuridine induces oxidative stress and activator protein-1 DNA binding activity in the embryo
Authors: Sahambi SK, Hales BF.
Journal: Birth Defects Res A Clin Mol Teratol (2006): 580
Authors: Sahambi SK, Hales BF.
Journal: Birth Defects Res A Clin Mol Teratol (2006): 580
The evaluation of early embryonic neurogenesis after exposure to the genotoxic agent 5-bromo-2′-deoxyuridine in mice
Authors: Kuwagata M, Ogawa T, Nagata T, Shioda S.
Journal: Neurotoxicology. (2006)
Authors: Kuwagata M, Ogawa T, Nagata T, Shioda S.
Journal: Neurotoxicology. (2006)
Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5′-bromo-2′-deoxyuridine
Authors: Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF.
Journal: Am J Physiol Cell Physiol (2005): C757
Authors: Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF.
Journal: Am J Physiol Cell Physiol (2005): C757
5-Bromo-2′-deoxyuridine is selectively toxic to neuronal precursors in vitro
Authors: Caldwell MA, He X, Svendsen CN.
Journal: Eur J Neurosci (2005): 2965
Authors: Caldwell MA, He X, Svendsen CN.
Journal: Eur J Neurosci (2005): 2965
Discrimination of cell nuclei in early S-phase, mid-to-late S-phase, and G(2)/M-phase by sequential administration of 5-bromo-2′-deoxyuridine and 5-chloro-2′-deoxyuridine
Authors: Yamada K, Semba R, Ding X, Ma N, Nagahama M.
Journal: J Histochem Cytochem (2005): 1365
Authors: Yamada K, Semba R, Ding X, Ma N, Nagahama M.
Journal: J Histochem Cytochem (2005): 1365
Neuropathological examination of fetal rat brain in the 5-bromo-2′-deoxyuridine-induced neurodevelopmental disorder model
Authors: Ogawa T, Kuwagata M, Muneoka KT, Shioda S.
Journal: Congenit Anom (Kyoto) (2005): 14
Authors: Ogawa T, Kuwagata M, Muneoka KT, Shioda S.
Journal: Congenit Anom (Kyoto) (2005): 14
Locomotor hyperactivity following prenatal exposure to 5-bromo-2′-deoxyuridine: neurochemical and behavioral evidence of dopaminergic and serotonergic alterations
Authors: Kuwagata M, Muneoka KT, Ogawa T, Takigawa M, Nagao T.
Journal: Toxicol Lett (2004): 63
Authors: Kuwagata M, Muneoka KT, Ogawa T, Takigawa M, Nagao T.
Journal: Toxicol Lett (2004): 63