ReadiUse 预活化别藻蓝蛋白APC-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiUse 预活化别藻蓝蛋白APC价格 4245
产品规格

1 mg

产品货号

ReadiUse 预活化别藻蓝蛋白APC

产品参数
Ex (nm) 651 Em (nm) 660
分子量 溶剂
存储条件
产品概述

产品基本信息

产品名称:ReadiUse 预活化别藻蓝蛋白APC

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):713

 

产品介绍

ReadiUse 预活化别藻蓝蛋白APC是美国AAT Bioquest生产的蛋白标记染料,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。 与其他藻胆蛋白一样,APC是荧光的,具有高的吸收率和高量子效率。 它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 AAT Bioquest提供这种预活化的APC,以促进APC与抗体和其他蛋白质如链霉抗生物素蛋白和其他二级试剂的结合。 我们的预活化APC已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。 此外,我们的预活化APC通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供优质的ReadiUse 预活化别藻蓝蛋白APC。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

ReadiUse 预活化APC-iFluor 700 Tandem-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiUse 预活化APC-iFluor 700 Tandem价格 4957
产品规格

1 mg

产品货号

ReadiUse 预活化APC-iFluor 700 Tandem

产品参数
Ex (nm) 651 Em (nm) 710
分子量 ~105000 溶剂
存储条件
产品概述

产品基本信息

产品名称:ReadiUse 预活化APC-iFluor 700 Tandem

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):713

 

产品介绍

ReadiUse 预活化APC-iFluor 700 Tandem是美国AAT Bioquest生产的蛋白标记染料,PE-AlexaFluor®700是流式细胞仪中常用的一种颜色。它的主要吸收峰在565 nm,发射峰在720 nm。AAT Bioquest提供了这种预活化的PE-iFluor 700 ,以替代流行的PE-AlexaFluor®700 Tandem。与串联的PE-AlexaFluor®700相比,PE-iFluor 700更亮,FRET效率更高。它用于促进PE-AlexaFluor®700串联连接至抗体和其他蛋白质,例如链霉亲和素和其他辅助试剂。我们的预活化PE-iFluor 700 Tandem比传统繁琐SMCC的PE-AlexaFluor®700 Tandem染料具有更高的产率,并具有更好的流式细胞仪性能。金畔生物是AAT Bioquest的中国代理商,为您提供优质的ReadiUse 预活化APC-iFluor 700 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化APC-iFluor 750 Tandem Cat#2571

ReadiUse 预活化APC-iFluor 750 Tandem-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiUse 预活化APC-iFluor 750 Tandem价格 4957
产品规格

1 mg

产品货号

ReadiUse 预活化APC-iFluor 750 Tandem

产品参数
Ex (nm) 651 Em (nm) 793
分子量 ~105000 溶剂
存储条件
产品概述

产品基本信息

产品名称:ReadiUse 预活化APC-iFluor 750 Tandem

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):779

 

产品介绍

预活化APC-iFluor 750 Tandem是美国AAT Bioquest生产的蛋白标记染料,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。 与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。 我们的APC-iFluor 750 Tandem由于其相似的光谱特性,是APC-Cy7®和APC-AlexaFluor®750Tandem的绝佳替代品。 我们的APC-iFluor 750 Tandem比APC-Cy7®Tandem更具光稳定性。 ReadiUse 预活化APC-iFluor 750 Tandem是一种活化的APC蛋白,可以很容易地与抗体结合,比传统的APC具有更高的结合产率。金畔生物是AAT Bioquest的中国代理商,为您提供优质的ReadiUse 预活化APC-iFluor 750 Tandem。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化APC-iFluor 700 Tandem Cat#2570
ReadiUse 预活化RPE-iFluor 750 Tandem Cat#2578

预活化的APC-Cy5.5 Tandem ReadiUse-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
预活化的APC-Cy5.5 Tandem ReadiUse价格 4957
产品规格

1 mg

产品货号

预活化的APC-Cy5.5 Tandem ReadiUse

产品参数
Ex (nm) 651 Em (nm) 700
分子量 ~105000 溶剂
存储条件
产品概述

产品基本信息

产品名称:预活化的APC-Cy5.5 Tandem ReadiUse

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):700

 

产品介绍

预活化的APC-Cy5.5 Tandem ReadiUse是美国AAT Bioquest生产的蛋白标记染料,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC是荧光的,具有高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 APC-Cy5.5是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~700nm。 AAT Bioquest提供这种预活化的APC-Cy5.5,以促进APC-Cy5.5与抗体和其他蛋白质(如链霉抗生物素蛋白和其他二级试剂)的串联结合。我们的预活化APC-Cy5.5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy5.5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。 金畔生物是AAT Bioquest的中国代理商,为您提供优质的预活化的APC-Cy5.5 Tandem ReadiUse。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化PE-Cy5.5 Tandem Cat#2581
串联染料PE-Cy5.5 Cat#2613
串联染料APC-Cy5.5 Cat#2622

ReadiUse 预活化APC-Cy7串联染料-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiUse 预活化APC-Cy7串联染料价格 4957
产品规格

1 mg

产品货号

ReadiUse 预活化APC-Cy7串联染料

产品参数
Ex (nm) 651 Em (nm) 779
分子量 ~105000 溶剂
存储条件
产品概述

产品基本信息

产品名称:ReadiUse 预活化APC-Cy7串联染料

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):780

 

产品介绍

ReadiUse 预活化APC-Cy7串联染料是美国AAT Bioquest生产的蛋白标记染料,别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 APC-Cy7是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~780nm。 AAT Bioquest提供这种预活化的APC-Cy7,以促进APC-Cy7串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化APC-Cy7串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy7串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供优质的ReadiUse 预活化APC-Cy7串联染料。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
ReadiUse 预活化PE-Cy7 Tandem Cat#2582

串联染料APC-Cy5.5-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
串联染料APC-Cy5.5价格 2823
产品规格

1 mg

产品货号

串联染料APC-Cy5.5

产品参数
Ex (nm) 651 Em (nm) 700
分子量 ~105000 溶剂 Water
存储条件 在2-8度冷藏保存, 避免光照
产品概述

产品基本信息

产品名称:串联染料APC-Cy5.5

储存条件:2-8℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

分子量:N/A

外观:固体

溶剂:水

激发波长(nm):651

发射波长(nm):700

 

产品介绍

串联染料APC-Cy5.5是美国AAT Bioquest生产的荧光染料。别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 APC-Cy5.5是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~700nm。 AAT Bioquest还提供独特的预活化APC-Cy5.5,以促进APC-Cy5.5与抗体和其他蛋白质(如链霉抗生物素蛋白和其他二级试剂)的串联结合。我们的预活化APC-Cy5.5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy5.5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商,为您提供优质的串联染料APC-Cy5.5。

点击查看光谱 

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
串联染料PE-Cy5.5 Cat#2613

串联染料APC-Cy7-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
串联染料APC-Cy7价格 2823
产品规格

1 mg

产品货号

串联染料APC-Cy7

产品参数
Ex (nm) 651 Em (nm) 779
分子量 ~105000 溶剂 Water
存储条件 在2-8度冷藏保存, 避免光照
产品概述

产品基本信息

产品名称:串联染料APC-Cy7

储存条件:2-8℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

分子量:N/A

外观:固体

溶剂:水

激发波长(nm):651

发射波长(nm):780

 

产品介绍

串联染料APC-Cy7是美国AAT Bioquest生产的荧光染料。别藻蓝蛋白(APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC是荧光的,具有极高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。APC-Cy7是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~780nm。AAT Bioquest还提供独特的预活化APC-Cy7,以促进APC-Cy7串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化APC-Cy7串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。金畔生物是AAT Bioquest的中国代理商,为您提供优质的串联染料APC-Cy7。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

ACQUITY APC XT BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K – 2M, 1/pk

产品货号: 186007252 产品名称: ACQUITY APC XT BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K – 2M, 1/pk 产品品牌: Waters

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 300,000 – 2,000,000 g/mole。

详细介绍

产品说明

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 300,000 – 2,000,000 g/mole。

规格

ACQUITY APC XT BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K - 2M, 1/pk

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 75 mm, 3K – 70K, 1/pk

产品货号: 186007003 产品名称: ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 75 mm, 3K – 70K, 1/pk 产品品牌: Waters

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mole。

详细介绍

产品说明

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mole。

规格

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 75 mm, 3K - 70K, 1/pk

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 30 mm, 3K – 70K, 1/pk

产品货号: 186007002 产品名称: ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 30 mm, 3K – 70K, 1/pk 产品品牌: Waters

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mole。

详细介绍

产品说明

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mole。

规格

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 30 mm, 3K - 70K, 1/pk

ACQUITY APC AQ BEH Column, 45Å, 1.7 µm, 4.6 mm X 30 mm, 200 – 5K, 1/pk

产品货号: 186006972 产品名称: ACQUITY APC AQ BEH Column, 45Å, 1.7 µm, 4.6 mm X 30 mm, 200 – 5K, 1/pk 产品品牌: Waters

ACQUITY APC AQ色谱柱旨在为水溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 200 – 5000 g/mole。

详细介绍

产品说明

ACQUITY APC AQ色谱柱旨在为水溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 200 – 5000 g/mole。

规格

ACQUITY APC AQ BEH Column, 45Å, 1.7 µm, 4.6 mm X 30 mm, 200 - 5K, 1/pk

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 150 mm, 3K – 70K, 1/pk

产品货号: 186007005 产品名称: ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 150 mm, 3K – 70K, 1/pk 产品品牌: Waters

ACQUITY APC XT Column(ACQUITY APC XT 色谱柱)经过优化,在有机溶剂可溶性聚合物表征过程中可进行基于分子量的高效分离。 该吸附剂采用亚乙基桥杂化 (BEH) 技术得到具有机械强度的填料床,抗收缩和溶胀,便于切换流动相溶剂。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mol。

详细介绍

产品说明

ACQUITY APC XT Column(ACQUITY APC XT 色谱柱)经过优化,在有机溶剂可溶性聚合物表征过程中可进行基于分子量的高效分离。 该吸附剂采用亚乙基桥杂化 (BEH) 技术得到具有机械强度的填料床,抗收缩和溶胀,便于切换流动相溶剂。该色谱柱的预期分子量范围约为 3,000 – 70,000 g/mol。

规格

ACQUITY APC XT BEH Column, 200Å, 2.5 µm, 4.6 mm X 150 mm, 3K - 70K, 1/pk

ACQUITY APC XT BEH Column, 45Å, 1.7 µm, 4.6 mm X 150 mm, 200 – 5K, 1/pk

产品货号: 186006995 产品名称: ACQUITY APC XT BEH Column, 45Å, 1.7 µm, 4.6 mm X 150 mm, 200 – 5K, 1/pk 产品品牌: Waters

ACQUITY APC XT 色谱柱经过优化,在有机溶剂可溶性聚合物表征过程中可进行基于分子量的高效分离。 该吸附剂采用亚乙基桥杂化 (BEH) 技术得到具有机械强度的填料床,抗收缩和溶胀,便于切换流动相溶剂。该色谱柱的预期分子量范围约为 200 – 5, 000 g/mol。

详细介绍

产品说明

ACQUITY APC XT 色谱柱经过优化,在有机溶剂可溶性聚合物表征过程中可进行基于分子量的高效分离。 该吸附剂采用亚乙基桥杂化 (BEH) 技术得到具有机械强度的填料床,抗收缩和溶胀,便于切换流动相溶剂。该色谱柱的预期分子量范围约为 200 – 5, 000 g/mol。

规格

ACQUITY APC XT BEH Column, 45Å, 1.7 µm, 4.6 mm X 150 mm, 200 - 5K, 1/pk

ACQUITY APC AQ BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K – 2M, 1/pk

产品货号: 186007249 产品名称: ACQUITY APC AQ BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K – 2M, 1/pk 产品品牌: Waters

ACQUITY APC AQ色谱柱旨在为水溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 300, 000 – 2,000, 000 g/mole。

详细介绍

产品说明

ACQUITY APC AQ色谱柱旨在为水溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 300, 000 – 2,000, 000 g/mole。

规格

ACQUITY APC AQ BEH Column, 900 Å, 2.5 µm, 4.6 mm X 30 mm, 300K - 2M, 1/pk

ACQUITY APC XT BEH Column, 450 Å, 2.5 µm, 4.6 mm X 75 mm, 20K – 400K, 1/pk Molecular Weight Max: 400000

产品货号: 186007008 产品名称: ACQUITY APC XT BEH Column, 450 Å, 2.5 µm, 4.6 mm X 75 mm, 20K – 400K, 1/pk Molecular Weight Max: 400000 产品品牌: Waters

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 20,000 – 400,000 g/mole。

详细介绍

产品说明

ACQUITY APC XT 色谱柱经过优化,可为有机溶剂可溶性聚合物表征提供基于尺寸的高效分离。该吸附剂采用乙烯桥混合 (BEH) 技术,提供机械坚固的填充床,可防止闪亮和膨胀,便于流动相溶剂切换。该色谱柱的预期分子量范围约为 20,000 – 400,000 g/mole。

规格

ACQUITY APC XT BEH Column, 450 Å, 2.5 µm, 4.6 mm X 75 mm, 20K - 400K, 1/pk Molecular Weight Max: 400000