标签归档：甲酯

D-荧光素甲酯-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
D-荧光素甲酯价格 2823
产品规格

5 mg

产品货号

D-荧光素甲酯

产品参数
Ex (nm) 385 Em (nm) 529
分子量 294.35 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称：D-荧光素甲酯

CAS：73918-26-0

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：294.35

溶剂：DMSO

激发波长(nm)：328

发射波长(nm)：533

 

产品介绍

D-荧光素甲酯是美国AAT Bioquest生产的荧光染料，D-荧光素甲酯含有在羧基位置连接的甲基部分，因此防止它被萤火虫荧光素酶识别。 然而，酯通过脂肪酶活性有效地水解，并且所得的D-荧光素被荧光素酶很好地识别。 D-荧光素甲酯是用于在均相测定中或在与荧光素酶及其辅因子组合的细胞裂解物样品中化学发光测量脂肪酶活性的敏感底物。金畔生物是AAT Bioquest的中国代理商，为您提供优质的D-荧光素甲酯。 

Luc的Ex/Em及其他相关内容链接：Spectral properties of luciferin and EGFP. A: Normalized excitation and… | Download Scientific Diagram (researchgate.net)

金畔问号：荧光素酶到底该如何检测？

 

试剂应用文献

Inhibition of drug-metabolizing enzymes by Jingyin granules: implications of herb–drug interactions in antiviral therapy
Authors: Zhang, Feng and Liu, Wei and Huang, Jian and Chen, Qi-long and Wang, Dan-dan and Zou, Li-wei and Zhao, Yong-fang and Zhang, Wei-dong and Xu, Jian-guang and Chen, Hong-zhuan and others,
Journal: Acta Pharmacologica Sinica (2021): 1–10
 
Inhibition of drug-metabolizing enzymes by Qingfei Paidu decoction: Implication of herb-drug interactions in COVID-19 pharmacotherapy
Authors: Zhang, Feng and Huang, Jian and Liu, Wei and Wang, Chao-Ran and Liu, Yan-Fang and Tu, Dong-Zhu and Liang, Xin-Miao and Yang, Ling and Zhang, Wei-Dong and Chen, Hong-Zhuan and others,
Journal: Food and Chemical Toxicology (2021): 111998
 
Carboxylesterase Activities and Protein Expression in Rabbit and Pig Ocular Tissues
Authors: Hammid, Anam and Fallon, John K and Lassila, Toni and Salluce, Giulia and Smith, Philip C and Tolonen, Ari and Sauer, Achim and Urtti, Arto and Honkakoski, Paavo
Journal: Molecular pharmaceutics (2021): 1305–1316
 
Human carboxylesterase 1A plays a predominant role in the hydrolytic activation of remdesivir in humans
Authors: Zhang, Feng and Li, Hong-Xin and Zhang, Tian-Tian and Xiong, Yuan and Wang, Hao-Nan and Lu, Zhen-Hui and Xiong, Lei and He, Yu-Qi and Ge, Guang-Bo
Journal: Chemico-biological interactions (2021): 109744
 
Rapalogues as hCES2A Inhibitors: In Vitro and In Silico Investigations
Authors: Shi, Cheng-Cheng and Song, Yun-Qing and He, Rong-Jing and Guan, Xiao-Qing and Song, Li-Lin and Chen, Shi-Tong and Sun, Meng-Ru and Ge, Guang-Bo and Zhang, Li-Rong
Journal: European Journal of Drug Metabolism and Pharmacokinetics (2020): 1–11
 
Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres–0497
 
C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
 
Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969–1974
 
Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969–1974
 
Discovery of novel adenylyl cyclase inhibitor by cell-based screening
Authors: Mano, Hiroki and Ishimoto, Tetsuya and Okada, Takuya and Toyooka, Naoki and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2014): 1689–1693

 

参考文献

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance
Authors: Law GH, G and elman OA, Tisi LC, Lowe CR, Murray JA.
Journal: Biochem J (2006): 305
 
Determination of p-glycoprotein ATPase activity using luciferase
Authors: Matsunaga T, Kose E, Yasuda S, Ise H, Ikeda U, Ohmori S.
Journal: Biol Pharm Bull (2006): 560
 
Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
Authors: Doyle TC, Nawotka KA, Purchio AF, Akin AR, Francis KP, Contag PR.
Journal: Microb Pathog (2006): 69
 
PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice
Authors: Wang JQ, Pollok KE, Cai S, Stantz KM, Hutchins GD, Zheng QH.
Journal: Bioorg Med Chem Lett (2006): 331
 
Bioluminescence of monolayers of firefly luciferase immobilized on graphite
Authors: Palomba S, Berovic N, Palmer RE.
Journal: Langmuir (2006): 5451
 
Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
Authors: Viviani VR, Ohmiya Y.
Journal: Luminescence (2006): 262
 
Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
Authors: Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.
Journal: Neurosurgery (2006): 365
 
Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)
 
Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Authors: Rodionova NS, Petushkov VN.
Journal: J Photochem Photobiol B (2006): 123
 
Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218

 

相关产品

产品名称 货号
D-荧光素乙酯 Cat#12515

Phytotech茉莉酸甲酯J389又一波来袭

发表于由

众所周知，茉莉酸和茉莉酸甲酯是植物中重要的激素之一，对植株体外源施加能够激发防御植物基因的表达，诱导植物的化学防御，在植株生长和发育过程中可调节植株叶片面积、花数、果实重量等，大致作用与脱落酸ABA相似，但是不同浓度的茉莉酸或者茉莉酸甲酯起到的作用也因植株不同而异。

研究发现，茉莉酸或者茉莉酸甲酯可能参与上调与蛋白酶抑制剂相关的基因，在植物生长发育过程中信号通路级联放大作用的泛素化作用有很大关系，涉及到多个基因的表达和多种蛋白质的作用，所以茉莉酸或者茉莉酸甲酯的作用机理与应用一直是大家研究和关注的重要方面。

Phytotech的茉莉酸甲酯（货号J389）自上市以来，产品性能和批间稳定性一直受到客户的青睐，但是为了给广大客户提供更加高品质的产品，在产品生产工艺上进行了改进，由于生产和测试时间较长，曾一度导致该产品缺货无法供应，截止目前，Phytotech的茉莉酸甲酯的升级产品已经重新上市，信息如下：

茉莉酸甲酯简介

货号：J389

品名：(+/-) Methyl Jasmonate

规格：5ml，25ml

Synonym: MeJA, 2-Pentenylcyclopentanone-3-acetic acid, Methyl 3-oxo-2-(pent-2-enyl)cyclopentaneacetate

CAS: 39924-52-2

Formula: C13H20O3

Molecular Wt: 224.3 g/mol

茉莉酸甲酯特性

Form: Liquid

Concentration: 10mg/ml

Appearance: Clear to Pale Yellow Liquid

Application: Plant Defense & Immunity

Solubility: Miscible with EtOH

Typical Working Concentration: 0.01-10 uM (depends on the application)

Storage Temp: 2 to 6 °C

Other Notes: Plant Tissue Culture Tested

Phytotech介绍：

  • 全球权威的专业植物组培试剂供应商；
  • 拥有强大的技术团队，超过30年的植物组培及产品开发经验；
  • 国际最早通过ISO 9001:2000质量体系认证植物组培公司；
  • 所有产品均遵照cGMP标准生产和包装，并全部经过植物组培验证；
  • 全系列组培产品，品种达1000多种；
  • 高性价比。

上海金畔生物科技有限公司（以下简称上海金畔生物科技有限公司）作为全球知名品牌推荐的优质代理商，以其开放、进取、诚信、专业等优势与国外多家著名试剂、原辅料和技术供应商建立了长期稳定的合作伙伴关系，力求为不同类型的客户提供前沿专业的解决方案以及优质的产品与高效的服务。

从2006 年开始，上海金畔生物科技有限公司与Phytotech 达成合作，成为中国地区独家代理，将优质试剂与服务传递给中国广大植物科学研究与生产企业客户，让您在使用顶级质量产品的同时，也享有低廉的价格。

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