产品基本信息

产品名称：5-DTAF [5-(4,6-二氯三嗪基)氨基荧光素]

CAS：51306-35-5

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：495.28

外观：黄色固体

溶剂：DMSO

激发波长（nm）：497

发射波长（nm）：516

产品介绍

荧光素二氯三嗪（5-DTAF）的5-异构体与蛋白质具有高度反应性。 与其他反应性荧光素不同，5-DTAF不仅与氨基反应，而且还与硫醇基团反应，甚至在pH大于9的水溶液中直接与羟基如多糖和其他醇反应。由于其高反应性，5- DTAF也用于标记碳水化合物。

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参考文献

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M.
Journal: Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O’Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253

Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167

Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215

Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hernandez M, Landfear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231

Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438

产品基本信息

产品名称：6-DTAF 

CAS：118357-32-7

储存条件：-15℃，避光干燥

保质期：12个月

物理化学光谱特性

分子量：495.28

溶剂：DMSO

Ex(nm):497

Em(nm):516

产品介绍

6-DTAF是美国AAT Bioquest生产的荧光染料，与5-DTAF相比，6-异构体可更好地用于寡核苷酸及核酸的标记。如同5-DTAF，6-DTAF不仅可与氨基发生反应，还能与巯基反应甚至可与羟基反应，如多糖及水溶液中pH值大于9的其它醇类直接反应。由于其高活性，6-DTAF也可用于标记碳水化合物。金畔生物是AAT Bioquest的中国代理商，为您提供优质的6-DTAF。 

点击查看光谱

参考文献

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M.
Journal: Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O’Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253

Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167

Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215

Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hernandez M, Landfear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231

Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438