死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2-AAT Bioquest荧光染料

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死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2价格 2823
产品规格

0.2 ml

产品货号

死细胞核染料 TWO-PRO 1  相当于TO-PRO 1  CAS 157199-59-2

产品参数
Ex (nm) 515 Em (nm) 531
分子量 645.38 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:死细胞核染料 TWO-PRO 1  相当于TO-PRO 1 

CAS:157199-59-2

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:645.38

激发波长(nm):515

发射波长(nm):531

 

产品介绍

TWO-PRO -1在化学上等同于TO-PRO®-1(TO-PRO®是Invitrogen的商标)。TWO-PRO -1是一种碳花青二聚体,具有类似于FITC的绿色荧光。它不具有细胞渗透性,可以很容易地与Cy5和罗丹明区别开来,作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的,并且在与DNA结合后会产生非常明亮的荧光信号。TWO-PRO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。同时使用细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的TWO-PRO -1进行标记,可用于评估细胞活力。TWO-PRO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。

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试剂应用文献

A toolkit for the identification of NEAT1_2/paraspeckle modulators
Authors: An, Haiyan and Elvers, Karen T and Gillespie, Jason A and Jones, Kimberley and Atack, John R and Grubisha, Olivera and Shelkovnikova, Tatyana A
Journal: Nucleic Acids Research (2022): e119–e119

 

参考文献

Daptomycin exerts rapid bactericidal activity against Bacillus anthracis without disrupting membrane integrity
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Journal: Acta Pharmacol Sin (2014): 211
 
Quantification of Candida albicans by flow cytometry using TO-PRO((R))-3 iodide as a single-stain viability dye
Authors: Kerstens M, Boulet G, Pintelon I, Hellings M, Voeten L, Delputte P, Maes L, Cos P.
Journal: J Microbiol Methods (2013): 189
 
A silicon cell cycle in a bacterial model of calcium phosphate mineralogenesis
Authors: Linton KM, Tapping CR, Adams DG, Carter RD, Shore RC, Aaron JE.
Journal: Micron (2013): 419
 
Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture
Authors: Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ.
Journal: J Physiol Pharmacol (2012): 537
 
Determination of the drug-DNA binding modes using fluorescence-based assays
Authors: Williams AK, Dasilva SC, Bhatta A, Rawal B, Liu M, Korobkova EA.
Journal: Anal Biochem (2012): 66
 
Transient changes in neuronal cell membrane permeability after blast exposure
Authors: Arun P, Abu-Taleb R, Valiyaveettil M, Wang Y, Long JB, Nambiar MP.
Journal: Neuroreport (2012): 342
 
A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
Authors: Gorokhova E, Mattsson L, Sundstrom AM.
Journal: J Microbiol Methods (2012): 216
 
SOCS-3 antagonizes pro-apoptotic effects of TRAIL and resveratrol in prostate cancer cells
Authors: Horndasch M, Culig Z.
Journal: Prostate (2011): 1357
 
The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190
 
Preclinical evaluation of novel triphenylphosphonium salts with broad-spectrum activity
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Journal: PLoS One (2010)