Hampton Siliconized Glass Cover Slides

Hampton Siliconized Glass Cover Slides

ApplicationsHanging, sitting or sandwich drop crystallization
FeaturesHigh purity of glassSiliconized, hydrophobic glass surface0.22 mm & 0.96 mm glass thicknessThicker glass slides preserve temperature longer12 mm, 18 mm, & 22 mm glass diameterCircle & squareUV Compatible (>85% transmission)
12 x 0.22 mm Circle
18 x 0.22 mm Circle
22 x 0.22 mm Circle & Square
Description
Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510), Dow Corning 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.
The following slides are UV transmissable (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

图片

图片
Plain Glass Cover Slides
ApplicationsHanging, sitting or sandwich drop crystallization
Features
0.22 mm glass thickness
12 mm glass diameter
Hydrophilic glass surface
Circle & squareDescription
Plain, non-siliconized, hydrophilic glass cover slides. Useful for hanging drop, sitting drop, and sandwich drop vapor diffusion crystallization methods. The hydrophilic surface creates a drop which is more flat than a drop on a siliconized surface. This offers enhanced imaging for very small drops. Use Dow Corning® Vacuum Grease (HR3-510), Dow Corning® 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate.
HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)
图片

图片
HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)
PEN-VAC Kit
ApplicationsHolding and manipulating small, flat surfaces such as glass cover slides
FeaturesSelf-contained vacuumNo power neededCompactDescription
PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.
图片
HR3-251  PEN-VAC Kit

Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability
Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio
Silver Bullets • Silver Bullets Bio
上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。
Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development
 

Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品
Features
  Membrane protein sparse matrix screens
  Sparse matrix formula efficiently samples salts, polymers, organics and pH
  pH range 4.6 – 8.5
  Formulated for use with detergents
   Tube or Deep Well block format
   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.
MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.
The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.
The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Applications

Cryocrystallography  低温结晶学
Features

SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

Description
The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.
The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.
The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.
Compatible with the following Synchrotron Radiation Beamlines
North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1
Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU
CrystalCap™
CrystalCap
CrystalCap™
CrystalCap & Vial
CrystalCap™
CrystalCap
Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™

CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate

Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate
Applications

Sitting drop crystallization
Features

One drop per reservoir
SBS format
48 well plate
9 mm standard distance between wells
Drop volume: Up to 10 µl
Reservoir volume: 50 to 200 µl
Micro-numbering alongside drop volumes
Rigid plate structure
Wide partition walls between wells improve sealing
Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom
UV compatible (UVP)
Description
MRC Maxi 48-Well Crystallization Plate for Automated Optimization
The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.
The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.
Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.
MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow.
The MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.
The advantages of the MRC Maxi Crystallization plate – in brief
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.
Easy Viewing
The wells are wide conical.
Each well has a micro lens for perfect illumination.
Micro numbering readable under the microscope for each well.
The optically superior polymer (UVP) is UV transmissible.
Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape.
Very Rigid, Automation-Friendly Plate Design
The UVP polymer reduces through-plastic evaporation to a minimum.
SBS Standard
The plates are designed to the SBS standard and are compatible with all common holders.
9 mm distance from well-to-well within columns, 18 mm distance within rows.
Unique Polymer (UVP)
Ultra-low sample binding.
No static charging.
Recommended Volumes
Volumes validated for MRC Maxi are up to 10 µl of sample drop and 200 µl of the crystallization reagent.
CAT NO NAME DESCRIPTION
HR3-179 MRC Maxi 48-Well Crystallization Plate 10 plate case
HR3-180 MRC Maxi 48-Well Crystallization Plate 40 plate case

Hampton晶体删选试剂盒

Hampton晶体删选试剂
1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
HR2-080
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体
⑵ Optimize™- Organics (Volatile) 挥发性有机物
⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物
⑷ Optimize™- Salts 盐类
⑸ Optimize™- Buffers缓冲液
⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB
⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物
⑻ Optimize™ – Cryoprotectants
⑼ Optimize™ – Oils用于结晶的油类
⑽ Optimize™ – Silica Hydrogel硅胶
⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等

Hampton 蛋白结晶删选试剂– Index HT

Hampton 蛋白结晶删选试剂– Index HT
Applications
Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules
多样结晶试剂系统删选试剂盒,用于蛋白质,复合物,肽,核酸,和水溶性的小分子。
Features
 Developed at Hampton Research
Screens classic, contemporary, & modern crystallization reagents
Samples pH 3 to 9
Compatible with microbatch, vapor diffusion, & liquid diffusion methods
Specially formulated reagent zones:
1 .Traditional salts versus pH
2.Neutralized organic acids
3.High [salt] with low [polymer]
4.High [polymer] with low [salt]
5.Low ionic strength versus pH
6.PEG & Salt versus pH
7.PEG & Salt
 Tube or Deep Well block format

Description
Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format.
Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.
Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.
Index contains 96 unique reagents, 10 ml each.
Index HT contains 96 unique reagents in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C
Index • Index HT
Index • Index HT
Apo Kinase crystal. Initial hit from the Hampton Research Index Screen. Annie Hassell, Glaxo Smithkline.
Index • Index HT
Crystal of a heme-based sensor from Thermoanaerobacter Tengcongensis grown using the Hampton Research Index screen. Patricia Pellicena & John Kuriyan at University of California, Berkeley, USA.

CAT NO NAME DESCRIPTION
HR2-144 Index 10 ml, tube format
HR2-134 Index HT 1 ml, Deep Well block format

Hampton GRAS 5 • GRAS 6 Screens

Hampton GRAS 5 • GRAS 6 Screens
Applications
 GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary reagent, sampling pH 4.5 to 10
. Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.5 to 10; 8 unique buffers
Primary crystallization reagent:

GRAS 5: PEG 300, 400, MME 550, & 600
GRAS 6: PEG 1,000, MME 2,000, 3,350 & 4,000

 

Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description

GRAS Screen™ 5 and 6 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 5 and 6 has been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.
GRAS Screen 5 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 5 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 5 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.
GRAS Screen 6 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 6 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 6 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

Hampton 蛋白质结晶试剂和耗材介绍

特色

Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商,我们将竭诚为您服务,欢迎新老客户咨询。

Hampton research主要产品:

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop
Crystallization Reagents
定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商,该公司的产品目录是由上海金畔生物科技有限公司提供,该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类:Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test(PCT预结晶试验)
应用:结晶筛选,确定相应的样品浓度,PCT试验用来预结晶结晶筛选,以确定适当的蛋白质浓度

  1. Slice pH(PH切片)

应用:溶解度和结晶筛选

  1. Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

  1. Index

应用:不同的试剂系统的结晶屏幕的蛋白质复合物,多肽,核酸,水溶性小分子。

  1. Crystal Screen(晶体屏幕)

应用:
主屏幕的可溶性肽,蛋白质,核酸,和水溶性小分子。稀疏矩阵的加法屏幕
5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶
6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶
7.Crystal Screen? & Crystal Screen? HT 用于蛋白质,复合物,缩氨酸,核酸以及水溶性小分子的传统的经典结晶试剂
8.Crystal Screen 2?用于蛋白质,缩氨酸,核酸以及水溶性小分子的结晶
9.Crystal Screen Lite?用于蛋白质,缩氨酸和核酸的结晶试剂
10.Crystal Screen Cryo?用于蛋白质,缩氨酸,核酸的结晶
11.Grid ScreenTM用于蛋白质的结晶,分为七小类。
12.Quik Screen?用于可溶性蛋白的结晶
13.Quik Optimize?快速优化试剂,与Quik Screen? 或 custom screen配套使用
14.Low Ionic Strength Screen?用于完整的单克隆抗体,单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶
15.Nucleic Acid Mini Screen?用于核酸片段的结晶化
16.Natrix?用于核酸,蛋白核酸聚合物的结晶
17.MembFac?& MembFac? HT用于膜蛋白的结晶
18.Additive Screen?增强或改变样品可溶性的试剂
19.Detergent Screen?去垢剂
20.Heavy Atom Screens重原子结晶试剂
21.Izit Crystal Dye?蛋白染色试剂,用于区分蛋白晶体和盐晶体

第二类:Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类:Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体
2.Optimize-Organics (Volatile) 挥发性有机物
3.Optimize-Organics (Non-Volatile)非挥发性的有机物
4.Optimize-Salts 盐类
5.Optimize-Buffers缓冲液
6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB
7.Optimize-Reducing Agent还原剂-TCEP氢氯化物
8.Optimize-Cryoprotectants
9.Optimize-Oils用于结晶的油类
10.Optimize-Silica Hydrogel硅胶
11.Optimize-LM Agarose低熔点琼脂糖

第四类:StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类:Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
2.48 Well Crystallization Plates 48孔晶体板
3.72 Well Crystallization Plates72孔晶体板
4.96 Well Crystallization Plates 96孔晶体板
5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶
6.Cover Slides & Related Tools
7.Glass Sitting Drop Rods用于坐滴的结晶
8.Micro-Bridges?微桥-坐滴结晶的支撑物
9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板
10.Sealants, Sealing Grease, Oils, Applicator密封油脂,油及其它器具
11.Sealing Films, Tapes, Mats & Covers
12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化
13.Granada Crystallization Box

第六类:Tools, Seeding & Resin(结晶工具、树脂)

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool

第七类:Cryocrystallography

  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类:Capillary Mounts & Supplies(毛细管座椅支架供应)
第九类:Goniometer Heads & Supplies(测角头及用品)
第十类:Xenon Derivatization(氙气衍生物)
第十一类:Labels & Pens(标签和记录笔)
第十二类:Protein Crystallization Standards(蛋白质结晶的标准)
Hampton代理,Hampton上海代理,Hampton北京代理,Hampton广州代理,Hampton总代理,Hampton一级代理,Hampton特约代理,Hampton现货上海金畔生物科技有限公司Hampton专业代理。
Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton蛋白结晶工具及仪器

Hampton蛋白结晶工具及仪器
CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

图片

图片

图片

图片

图片

图片
Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
图片
CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)
图片

图片

图片
Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°
图片

CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool
图片

图片

Hampton Optimize Reagents

Hampton Optimize Reagents
1、Optimize – Polymers
Crystallization grade polymers for formulating screens or optimization
2、Optimize – Organics (Volatile)
Crystallization grade organics (volatile) for formulating screens or optimization
3、Optimize – Organics (Non-Volatile)
Crystallization grade organics (non-volatile) for formulating screens or optimization
4、Optimize – Salts
Crystallization grade salts for formulating screens or optimization
5、Optimize – Buffers
Crystallization grade buffers for formulating screens or optimization
6、Optimize – Solubilizing Agents (NDSB)
Crystallization grade non detergent sulfobetaines
7、Optimize – Reducing Agent
TCEP Hydrochloride
8、Optimize – Monoolein
LCP – Lipidic Cubic Phase
9、Optimize – Cryoprotectants
For cryoprotection of crystals
10、Optimize – Oils for Crystallization
Microbatch crystallization & oil layering
11、Optimize – Izit Crystal Dye
Izit™ color dye to differentiate protein crystals from salt crystals

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Applications

Cryocrystallography  低温结晶学
Features

SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

Description
The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.
The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.
The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.
Compatible with the following Synchrotron Radiation Beamlines
North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1
Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU
CrystalCap™
CrystalCap
CrystalCap™
CrystalCap & Vial
CrystalCap™
CrystalCap
Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™

CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Hampton 水晶透明密封带Crystal Clear Sealing Tape
Crystal Clear Sealing Tape
3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)
Crystal Clear Sealing Tape
0.75 inch Crystal Clear Sealing Tape
Applications  应用
Sealing tape used to seal sitting drop crystallization experiments
Description
These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.
Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Applications

Cryocrystallography
Features

Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.
Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.
The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.
Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.
The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic
18 mm Mounted CryoLoop™ - 20 micron
Crystal in CryoLoop

CAT NO NAME DESCRIPTION
HR4-932 18 mm Mounted CryoLoop – 20 micron 0.025 – 0.05 mm – 25 pack
HR4-945 18 mm Mounted CryoLoop – 20 micron 0.05 – 0.1 mm – 25 pack
HR4-947 18 mm Mounted CryoLoop – 20 micron 0.1 – 0.2 mm – 25 pack
HR4-970 18 mm Mounted CryoLoop – 20 micron 0.2 – 0.3 mm – 25 pack
HR4-971 18 mm Mounted CryoLoop – 20 micron 0.3 – 0.4 mm – 25 pack
HR4-972 18 mm Mounted CryoLoop – 20 micron 0.4 – 0.5 mm – 25 pack
HR4-973 18 mm Mounted CryoLoop – 20 micron 0.5 – 0.7 mm – 25 pack
HR4-974 18 mm Mounted CryoLoop – 20 micron 0.7 – 1.0 mm – 25 pack
HR4-918 18 mm Mounted CryoLoop – 20 micron Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

Hampton结晶用缓冲盐试剂盒– SaltRx

Hampton结晶用缓冲盐试剂盒– SaltRx
SaltRx 1 • SaltRx 2 • SaltRx HT

Applications
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Features

Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
 Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
CAT NO NAME DESCRIPTION
HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format