Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Applications

Cryocrystallography
Features

Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.
Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.
The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.
Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.
The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic
18 mm Mounted CryoLoop™ - 20 micron
Crystal in CryoLoop

CAT NO NAME DESCRIPTION
HR4-932 18 mm Mounted CryoLoop – 20 micron 0.025 – 0.05 mm – 25 pack
HR4-945 18 mm Mounted CryoLoop – 20 micron 0.05 – 0.1 mm – 25 pack
HR4-947 18 mm Mounted CryoLoop – 20 micron 0.1 – 0.2 mm – 25 pack
HR4-970 18 mm Mounted CryoLoop – 20 micron 0.2 – 0.3 mm – 25 pack
HR4-971 18 mm Mounted CryoLoop – 20 micron 0.3 – 0.4 mm – 25 pack
HR4-972 18 mm Mounted CryoLoop – 20 micron 0.4 – 0.5 mm – 25 pack
HR4-973 18 mm Mounted CryoLoop – 20 micron 0.5 – 0.7 mm – 25 pack
HR4-974 18 mm Mounted CryoLoop – 20 micron 0.7 – 1.0 mm – 25 pack
HR4-918 18 mm Mounted CryoLoop – 20 micron Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

Hampton结晶用缓冲盐试剂盒– SaltRx

Hampton结晶用缓冲盐试剂盒– SaltRx
SaltRx 1 • SaltRx 2 • SaltRx HT

Applications
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Features

Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
 Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
CAT NO NAME DESCRIPTION
HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)
VDX Plate with sealant
Applications

Hanging drop crystallization
Sitting drop crystallization with Micro-Bridges® or Glass Sitting Drop Rods™
Dialysis crystallization with Dialysis Buttons™
Features

24 well plate with raised cover
Sealant applied to 24 wells, ready to seal with cover slides
Compatible with 22 mm diameter square and circle cover slides
Optically clear plastic
Extremely versatile and cost-effective crystallization platform
Compatible with UV crystal imaging when used with HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides and HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

Description
24 well crystallization plate for hanging drop or sitting drop vapor diffusion crystallization (when used with Micro-Bridges or Glass Rods), or dialysis crystallization (when used with Dialysis Buttons). Stackable, optically clear plastic 24 well plates with raised covers (to allow room for cover slides) and flat bottoms for exceptional optics. Raised, wide rings around each reservoir (well) minimize cross contamination and allow each well to be individually sealed with 22 mm diameter circle or square cover slides. Plates are individually wrapped and supplied with applied sealant. 15.0 cm x 10.8 cm plate footprint is convenient for manual pipetting and well access while at the same time compatible with some automated liquid handling systems. Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 500 to 1,000 µl. Well capacity: 3.5 ml.

CAT NO NAME DESCRIPTION
HR3-172 VDX邃「 Plate with sealant 24 plate case
HR3-170 VDX邃「 Plate with sealant 100 plate case

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Applications

Cryocrystallography  低温结晶学
Features

SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

Description
The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.
The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.
The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.
Compatible with the following Synchrotron Radiation Beamlines
North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1
Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU
CrystalCap™
CrystalCap
CrystalCap™
CrystalCap & Vial
CrystalCap™
CrystalCap
Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™

CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

Hampton GRAS 3 • GRAS 4 Screens

Hampton GRAS 3 • GRAS 4 Screens
Applications
a.GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Salt is the primary, and Polyethylene glycol the secondary reagent, sampling pH 4-9
b.Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

24 unique Salts

 

Secondary crystallization reagent:

GRAS Screen 3: PEG 300, 400, MME 550, & 600
GRAS Screen 4: PEG 1,000, MME 2,000, 3,350 & 4,000

 

Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

 Description
GRAS Screen™ 3 and GRAS Screen™ 4 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 3 and 4 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 3 samples twenty four salts, encompassing pH 4-9 versus four low molecular weight Polyethylene glycols (300, 400, MME 550, & 600). GRAS Screen 3 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 3 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.
GRAS Screen 4 samples twenty four salts, encompassing pH 4-9 versus four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350 & 4,000). GRAS Screen 4 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 4 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.
 

CAT NO NAME DESCRIPTION
HR2-453 GRAS Screen 3 1 ml, Deep Well block format
HR2-454 GRAS Screen 4 1 ml, Deep Well block format

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate
美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
Hampton research主要产品:
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂
Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate
HDP Hanging Drop Crystallization Plate (Swissci)
HDP Hanging Drop Plate
HDP Hanging Drop Crystallization Plate (Swissci)
Single Well with Tool
HDP Hanging Drop Crystallization Plate (Swissci)
Hanging Drop Wells
Applications—Hanging drop crystallization
Features
  Made from optically superior polymer (UVP)
  Allen key novel crystal plug closure system
  Wells are micro-numbered
Description  描述:
The Swissci Hanging Drop Crystallization Plate is a breakthrough for protein crystallization in a 96-well format. For the first time individual wells can be identified and removed without any disturbance to the growing crystals inside the plate.Description
The plate has been developed by Swissci AG together with leading Crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization.
The Hanging Drop Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval. The system is fully covered by design and patent protection.
The Advantages of the Hanging Drop Crystallization Plate
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy. Allen key system allows for smooth removal and subsequent microscopic investigation / X-ray with the novel crystal plug closure all included in the system.
Easy Viewing
The wells are wide conical and have a lens effect for perfect illumination. The micro-numbering ensures you will never loose the location under the microscope.
The optically superior polymer is UV transmissible and can be used to differentiate between salt and protein crystals.
Integral Sealing
Wide partition walls between each well provides a high surface area for very good sealing with the advanced quality sealing tape. The sealing tape is integrated into the kit so there is no need to purchase additional parts prior to starting a procedure.
Wide Range of Volumes
Typical volumes are 50 – 200 µl of reservoir and 10 nanoliter – 5 microliter drop size.
SBS Standard
The complete plate is designed to the 96-well SBS standard for all common holders.

CAT NO NAME DESCRIPTION
HR3-187 Hanging Drop Crystallization Plate (Swissci) 10 plate case

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate 相关产品:

96 Well Hanging Drop Vapor Diffusion Plate
Hanging drop crystallization
ARI Hanging Drop Seals for 96 Well Plates
ARI Hanging Drop Seals for 96 Well Plates
CrystalQuick 96 Well Sitting Drop Plate (Greiner)
Sitting drop crystallization
Detergent Screen HT
96 detergents Deep Well block format
Glass Cover Slide Gizmo Dispenser
Cover Slide Dispenser
Greiner ComboPlate and CrystalBridge
Hanging and sitting drop crystallization
HDP Hanging Drop Crystallization Plate (Swissci)
Hanging Drop Crystallization
Linbro Plate
Hanging drop crystallization
ProCrystal
Protein Crystallization Covers for Hanging Drop Vapor Diffusion
Protein Methods
Daniel M. Bollag, Michael D. Rozycki, & Stuart J. Edelstein
Solubility & Stability Screen
Identify protein specific additives that promote solubility and stability
UVP Hanging Drop Plate Seal (Swissci)
Swissci UVP Hanging Drop Plate Seal
VDX Plate with sealant
Hanging drop crystallization
VDX Plate without sealant
Hanging drop crystallization
VDX48 Plate with sealant
Hanging drop crystallization
VDXm Plate without sealant
Hanging drop crystallization
VDXm Plate with sealant
Hanging drop crystallization

 

Hampton Polyethylene glycol 4,000

Hampton Polyethylene glycol 4,000

Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

CAT NO NAME DESCRIPTION
HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications
  Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
Features

Developed at Hampton Research
PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
 Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.
PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™

Applications

Cryocrystallography  低温结晶学
Features

SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

Description
The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.
The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.
The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.
Compatible with the following Synchrotron Radiation Beamlines
North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1
Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU
CrystalCap™
CrystalCap
CrystalCap™
CrystalCap & Vial
CrystalCap™
CrystalCap
Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™

CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

Hampton 蛋白结晶试剂–Crystal Screen

Hampton 蛋白结晶试剂–Crystal Screen
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Applications
 Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
 Sparse matrix additive screen
Features
 The original sparse matrix screen
 Sparse matrix formula efficiently samples salts, polymers, organics, & pH
 Proven effective with more than 1,000 biological macromolecules
 Tube or Deep Well block format

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.
A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.
Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.
Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.
Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

CAT NO NAME DESCRIPTION
HR2-110 Crystal Screen 10 ml, tube format
HR2-112 Crystal Screen 2 10 ml, tube format
HR2-130 Crystal Screen HT 1 ml, Deep Well block format

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Applications

Cryocrystallography
Features

Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.
Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.
The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.
Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.
The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop
18 mm Mounted CryoLoop™ - 20 micron
18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic
18 mm Mounted CryoLoop™ - 20 micron
Crystal in CryoLoop

CAT NO NAME DESCRIPTION
HR4-932 18 mm Mounted CryoLoop – 20 micron 0.025 – 0.05 mm – 25 pack
HR4-945 18 mm Mounted CryoLoop – 20 micron 0.05 – 0.1 mm – 25 pack
HR4-947 18 mm Mounted CryoLoop – 20 micron 0.1 – 0.2 mm – 25 pack
HR4-970 18 mm Mounted CryoLoop – 20 micron 0.2 – 0.3 mm – 25 pack
HR4-971 18 mm Mounted CryoLoop – 20 micron 0.3 – 0.4 mm – 25 pack
HR4-972 18 mm Mounted CryoLoop – 20 micron 0.4 – 0.5 mm – 25 pack
HR4-973 18 mm Mounted CryoLoop – 20 micron 0.5 – 0.7 mm – 25 pack
HR4-974 18 mm Mounted CryoLoop – 20 micron 0.7 – 1.0 mm – 25 pack
HR4-918 18 mm Mounted CryoLoop – 20 micron Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

图片
Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.
图片
CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.
图片

Hampton 0.1毫米50号玻璃毛细管

Hampton 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌:Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用
X射线数据采集
液-液扩散结晶
凝胶针状结晶
特征
50号硼硅玻璃
壁厚 10微米
描述
硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。
请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。
0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。
 

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability
Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio
Silver Bullets • Silver Bullets Bio
上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。
Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development
 

Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品
Features
  Membrane protein sparse matrix screens
  Sparse matrix formula efficiently samples salts, polymers, organics and pH
  pH range 4.6 – 8.5
  Formulated for use with detergents
   Tube or Deep Well block format
   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.
MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.
The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.
The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format